Linked Life Data

7797518

Source:http://linkedlifedata.com/resource/pubmed/id/7797518

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1995-7-31
pubmed:abstractText
Among the primate lentiviruses (human immunodeficiency virus (HIV) -1, HIV-2, and simian immunodeficiency virus (SIV), the nef gene is highly conserved and encodes a myristylated protein of approximately 27 kDa (HIV-1) or approximately 34 kDa (HIV-2, SIV). Previously, we found Nef expressed either as a CD8-Nef fusion protein or as a native protein in virally infected T cell lines associates with a cellular serine kinase. This kinase activity phosphorylated two proteins of 62 and 72 kDa that coimmunoprecipitate with Nef in in vitro kinase assays. Using transient expression, various Nef alleles and mutants have been analyzed for association with the cellular kinase activity. The ability of Nef to associate with the kinase activity is conserved among several alleles of HIV-1 as well as SIVmac239 and is observed in non-lymphoid cell lines of simian and murine origins. Two separate regions of HIV-1SF2 Nef are critical for the associated kinase activity. One domain overlaps with a central highly conserved region found in all primate lentivirus nef genes and has been provisionally mapped to amino acids 45-127. Because membrane localization of Nef is important for the associated cellular kinase activity, the second domain represents a membrane targeting signal. Moreover, point mutations within the central region that abrogate the Nef-associated kinase activity in HIV-1SF2 Nef have the same effect when introduced into SIVmac239open Nef.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
270
pubmed:geneSymbol
nef
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15307-14
pubmed:dateRevised
2007-11-19
pubmed:meshHeading
pubmed-meshheading:7797518-Amino Acid Sequence, pubmed-meshheading:7797518-Animals, pubmed-meshheading:7797518-Cell Line, pubmed-meshheading:7797518-Cercopithecus aethiops, pubmed-meshheading:7797518-Conserved Sequence, pubmed-meshheading:7797518-Fibroblasts, pubmed-meshheading:7797518-Gene Products, nef, pubmed-meshheading:7797518-Genes, nef, pubmed-meshheading:7797518-HIV-1, pubmed-meshheading:7797518-HIV-2, pubmed-meshheading:7797518-Humans, pubmed-meshheading:7797518-Kidney, pubmed-meshheading:7797518-Molecular Sequence Data, pubmed-meshheading:7797518-Mutagenesis, Site-Directed, pubmed-meshheading:7797518-Primates, pubmed-meshheading:7797518-Protein-Serine-Threonine Kinases, pubmed-meshheading:7797518-Sequence Homology, Amino Acid, pubmed-meshheading:7797518-Simian immunodeficiency virus, pubmed-meshheading:7797518-Transfection, pubmed-meshheading:7797518-nef Gene Products, Human Immunodeficiency Virus, pubmed-meshheading:7797518-p21-Activated Kinases
pubmed:year
1995
pubmed:articleTitle
A conserved domain and membrane targeting of Nef from HIV and SIV are required for association with a cellular serine kinase activity.
pubmed:affiliation
Department of Medicine, University of California, San Francisco 94143-0128, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't