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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
25
|
| pubmed:dateCreated |
1995-7-31
|
| pubmed:abstractText |
To assess the mechanisms controlling the developmental regulation of tropoelastin expression in vivo, we developed a reverse-transcription-polymerase chain reaction (RT-PCR) assay to detect tropoelastin pre-mRNA as an indicator of ongoing transcription in intact tissue. RNA was isolated from mid-fetal (early-elastogenic), neonatal (peak tropoelastin expression), and adult (very low tropoelastin expression) rat lungs and reverse transcribed, and the cDNA was amplified with intron specific primers. A weak hybridization signal for tropoelastin pre-mRNA was seen in mid-fetal samples, and paralleling the increase in steady-state mRNA levels, a strong signal for pre-mRNA was detected in neonatal samples, indicating transcriptional regulation. Stimulation of fetal lung tropoelastin expression by maternal administration of dexamethasone also led to an increase in pre-mRNA levels. However, signal for tropoelastin pre-mRNA in adult samples was equal to that detected in neonatal samples, even though mRNA levels had dropped about 80-fold. Persistence of tropoelastin transcription in adult tissue was also seen in cell culture models and was verified by nuclear runoff assay. In addition, an RT-PCR assay for alpha 1 (I) procollagen pre-mRNA accurately revealed the known transcriptional regulation of this gene. Our results demonstrate that the induction and maintenance of elastogenesis is controlled by a transcriptional mechanism, whereas, the cessation of tropoelastin expression is controlled by a post-transcriptional mechanism.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Elastin,
http://linkedlifedata.com/resource/pubmed/chemical/Procollagen,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Tropoelastin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
23
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
14899-906
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7797468-Aging,
pubmed-meshheading:7797468-Animals,
pubmed-meshheading:7797468-Animals, Newborn,
pubmed-meshheading:7797468-Base Sequence,
pubmed-meshheading:7797468-Cell Nucleus,
pubmed-meshheading:7797468-Cells, Cultured,
pubmed-meshheading:7797468-DNA Primers,
pubmed-meshheading:7797468-Elastin,
pubmed-meshheading:7797468-Female,
pubmed-meshheading:7797468-Fibroblasts,
pubmed-meshheading:7797468-Gene Expression Regulation,
pubmed-meshheading:7797468-Lung,
pubmed-meshheading:7797468-Molecular Sequence Data,
pubmed-meshheading:7797468-Polymerase Chain Reaction,
pubmed-meshheading:7797468-Pregnancy,
pubmed-meshheading:7797468-Procollagen,
pubmed-meshheading:7797468-RNA, Messenger,
pubmed-meshheading:7797468-RNA Precursors,
pubmed-meshheading:7797468-Rats,
pubmed-meshheading:7797468-Rats, Sprague-Dawley,
pubmed-meshheading:7797468-Tropoelastin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Developmental regulation of elastin production. Expression of tropoelastin pre-mRNA persists after down-regulation of steady-state mRNA levels.
|
| pubmed:affiliation |
Department of Medicine (Dermatology), Jewish Hospital, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|