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pubmed-article:7797427pubmed:abstractTextAccurate ultrastructural localization of the components of centrosomes is an important step toward the determination of their function. We have used an electron microscopy procedure to preserve centrosome-associated antigens which enables their high-resolution localization. The unique part of our procedure is the application of a post-sectioning fixation step which overcomes the poor section contrast and morphological appearance that limits the use of low-temperature processing and Lowicryl embedding. The efficacy of our approach is demonstrated by the efficient labelling of alpha-tubulin in the well-preserved and contrasted microtubule barrels of the centrides of isolated mammalian centrosomes.lld:pubmed
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pubmed-article:7797427pubmed:dateRevised2004-11-17lld:pubmed
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pubmed-article:7797427pubmed:year1995lld:pubmed
pubmed-article:7797427pubmed:articleTitlePostembedding alpha-tubulin immunolabelling of isolated centrosomes.lld:pubmed
pubmed-article:7797427pubmed:affiliationStructural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.lld:pubmed
pubmed-article:7797427pubmed:publicationTypeJournal Articlelld:pubmed
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