Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-7-31
|
| pubmed:abstractText |
Accurate ultrastructural localization of the components of centrosomes is an important step toward the determination of their function. We have used an electron microscopy procedure to preserve centrosome-associated antigens which enables their high-resolution localization. The unique part of our procedure is the application of a post-sectioning fixation step which overcomes the poor section contrast and morphological appearance that limits the use of low-temperature processing and Lowicryl embedding. The efficacy of our approach is demonstrated by the efficient labelling of alpha-tubulin in the well-preserved and contrasted microtubule barrels of the centrides of isolated mammalian centrosomes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0018-2214
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
240-6
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7797427-Centrosome,
pubmed-meshheading:7797427-Epoxy Resins,
pubmed-meshheading:7797427-Humans,
pubmed-meshheading:7797427-Immunohistochemistry,
pubmed-meshheading:7797427-Lymphoid Tissue,
pubmed-meshheading:7797427-Microscopy, Electron,
pubmed-meshheading:7797427-Microtubules,
pubmed-meshheading:7797427-Plastic Embedding,
pubmed-meshheading:7797427-Tubulin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Postembedding alpha-tubulin immunolabelling of isolated centrosomes.
|
| pubmed:affiliation |
Structural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article
|