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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-8-3
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pubmed:databankReference |
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pubmed:abstractText |
A soluble aminotransferase with kynurenine aminotransferase (KAT) activity has been recently isolated from rat brain. This enzyme corresponds to a cytosolic form of glutamine transaminase K (GTK). In addition to the cytosolic enzyme, a mitochondrial-associated form of this KAT/GTK also exists. In the present work we have isolated a rat brain cDNA clone encoding a KAT/GTK enzyme identical to the soluble form but carrying an additional stretch of 32 amino acids at its NH2-terminus. Several structural features of this sequence resemble those of leader peptides for mitochondrial import. Evidence that the isolated cDNA encoded for mitochondrial KAT/GTK was obtained after transfection of HEK-293 cells with the cDNA coding for this new KAT/GTK isoenzyme. In fact, a significant enrichment of both KAT and GTK enzymatic activities was found in the crude mitochondrial fraction of the transfected cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
367
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7796908-Amino Acid Sequence,
pubmed-meshheading:7796908-Animals,
pubmed-meshheading:7796908-Base Sequence,
pubmed-meshheading:7796908-Brain,
pubmed-meshheading:7796908-Cell Line,
pubmed-meshheading:7796908-Cloning, Molecular,
pubmed-meshheading:7796908-DNA, Complementary,
pubmed-meshheading:7796908-Gene Expression,
pubmed-meshheading:7796908-Lyases,
pubmed-meshheading:7796908-Mitochondria,
pubmed-meshheading:7796908-Molecular Sequence Data,
pubmed-meshheading:7796908-Rats,
pubmed-meshheading:7796908-Transaminases,
pubmed-meshheading:7796908-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain.
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pubmed:affiliation |
Pharma Division, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
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pubmed:publicationType |
Journal Article
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