Linked Life Data

7795255

Source:http://linkedlifedata.com/resource/pubmed/id/7795255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-3
pubmed:abstractText
Structural alterations of the 5' noncoding region of the BCL-6 gene have been found in 40% of diffuse large cell lymphoma (DLCL) and 5% to 10% of follicular lymphomas (FL), suggesting that deregulated BCL-6 expression may play a role in lymphomagenesis. Nucleotide sequencing of BCL-6 cDNA predicted a protein containing six zinc-finger domains, suggesting that it may function as a transcription factor. Using antisera raised against N- and C-terminal BCL-6 synthetic oligopeptides in immunoprecipitation, immunoblot, and immunocytochemical assays, this study identifies the BCL-6 gene product as a 95-kD nuclear protein. Western blot analysis of human tumor cell lines representative of various hematopoietic lineages/stages of differentiation showed that the BCL-6 protein is predominantly expressed in the B-cell lineage where it was found in mature B cells. Immunohistochemical analysis of normal human lymphoid tissues indicated that BCL-6 expression is topographically restricted to germinal centers including all centroblasts and centrocytes. The BCL-6 protein was also detectable in inter- and intra-follicular CD4+ T cells, but not in other follicular components including mantle-zone B cells, plasma cells, dendritic cells, and macrophages. Immunohistochemical analysis of DLCL and FL biopsy samples showed that the BCL-6 protein is detectable in these tumors independent of the presence of BCL-6 gene rearrangements. These results indicate that the expression of the BCL-6 gene is specifically regulated during B-cell differentiation and suggest a role for BCL-6 in germinal center development or function. Because DLCL derive from germinal-center B cells, deregulated BCL-6 expression may contribute to lymphomagenesis by preventing postgerminal center differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7795255-Amino Acid Sequence, pubmed-meshheading:7795255-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:7795255-B-Lymphocyte Subsets, pubmed-meshheading:7795255-Bone Marrow Cells, pubmed-meshheading:7795255-Burkitt Lymphoma, pubmed-meshheading:7795255-Cell Differentiation, pubmed-meshheading:7795255-Cell Nucleus, pubmed-meshheading:7795255-DNA-Binding Proteins, pubmed-meshheading:7795255-Embryonal Carcinoma Stem Cells, pubmed-meshheading:7795255-Gene Expression Regulation, Neoplastic, pubmed-meshheading:7795255-Hematopoietic Stem Cells, pubmed-meshheading:7795255-Humans, pubmed-meshheading:7795255-Immunophenotyping, pubmed-meshheading:7795255-Lymph Nodes, pubmed-meshheading:7795255-Lymphoma, B-Cell, pubmed-meshheading:7795255-Lymphoma, Follicular, pubmed-meshheading:7795255-Lymphoma, Large B-Cell, Diffuse, pubmed-meshheading:7795255-Molecular Sequence Data, pubmed-meshheading:7795255-Neoplasm Proteins, pubmed-meshheading:7795255-Neoplastic Stem Cells, pubmed-meshheading:7795255-Palatine Tonsil, pubmed-meshheading:7795255-Proto-Oncogene Proteins, pubmed-meshheading:7795255-Proto-Oncogene Proteins c-bcl-6, pubmed-meshheading:7795255-Transcription Factors, pubmed-meshheading:7795255-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
BCL-6 protein is expressed in germinal-center B cells.
pubmed:affiliation
Department of Pathology, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't