Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-8-3
|
| pubmed:abstractText |
We investigated the rearrangement and expression of TCR genes in mouse fetal thymus organ culture, a system that avoids subsequent entry of hematopoietic precursor cells. The first observable rearranged TCR gene was homogeneous V gamma 2-J gamma 2, detectable as early as fetal day 11 (d11) in the thymic primordia. The productive TCR was homogeneous V gamma 5-J gamma 1, first detectable in d13 thymocytes, followed by adult-type TCR gamma (V gamma 4 and V gamma 7). Sequence analysis of TCR revealed five types of V-J junctional sequences. In the very early stage, a homogeneous V-J junction is generated via a short homology sequence in the coding region (Type I), while a short homology sequence in the P-nucleotide rather than the coding region is used in the following stage (Type II). In the later embryonic stages, diverse V-J junctions are generated by well-known mechanisms, such as P-nucleotide (Type III), N-region insertion (Type IV) or trimming of the coding ends (Type V). These findings suggest that the generation of homogeneous TCR gamma (V gamma 2 and V gamma 5) in the early fetal stages is due to the intrinsic rearrangement mechanisms and is in stage specific manner.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0953-8178
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:geneSymbol |
J<down>&ggr;</down>1,
J<down>&ggr;</down>2,
J<down>&ggr;</down>7,
V<down>&ggr;</down>2,
V<down>&ggr;</down>4,
V<down>&ggr;</down>5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
493-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7794825-Animals,
pubmed-meshheading:7794825-Base Sequence,
pubmed-meshheading:7794825-DNA, Circular,
pubmed-meshheading:7794825-DNA, Complementary,
pubmed-meshheading:7794825-Female,
pubmed-meshheading:7794825-Gene Expression Regulation, Developmental,
pubmed-meshheading:7794825-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:7794825-Male,
pubmed-meshheading:7794825-Mice,
pubmed-meshheading:7794825-Mice, Inbred BALB C,
pubmed-meshheading:7794825-Mice, Inbred C57BL,
pubmed-meshheading:7794825-Molecular Sequence Data,
pubmed-meshheading:7794825-Organ Culture Techniques,
pubmed-meshheading:7794825-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:7794825-Sequence Alignment,
pubmed-meshheading:7794825-Specific Pathogen-Free Organisms,
pubmed-meshheading:7794825-Thymus Gland
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
TCR repertoire in early fetal mouse thymus.
|
| pubmed:affiliation |
Division of Molecular Immunology, School of Medicine, Chiba University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|