Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-7-24
|
| pubmed:abstractText |
A blastogenesis microassay employing 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) was adapted to measure blastogenic responses of lymphocytes from the chicken's head-associated lymphoid tissues (i.e., the harderian gland and conjunctiva-associated lymphoid tissue) to T- and B-cell mitogens. Lymphocytes isolated from peripheral blood, spleen, and the harderian gland had highly significant (P < 0.01) responses to T- and B-cell mitogens compared with control lymphocytes cultured without mitogens. Cultured lymphocytes obtained from the harderian gland had highly significant mitogenic responses to the T-cell mitogen concanavalin A (25 to 100 micrograms/ml) and to the B-cell mitogen Salmonella typhimurium lipopolysaccharide (1.25 to 5.0 micrograms/ml) compared with the control lymphocytes. Mitogenic responses of cultured lymphocytes obtained from the conjunctiva-associated lymphoid tissue could not be measured within the given parameters of the blastogenesis microassay. This was primarily due to the low yield of lymphocytes, which proved to be a limiting factor. The ability of the MTT blastogenesis microassay to detect blastogenic responses of the harderian gland to mitogens may be indicative of its usefulness for measuring cell-mediated immunity responses to other antigens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0005-2086
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
39
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7794167-Animals,
pubmed-meshheading:7794167-B-Lymphocytes,
pubmed-meshheading:7794167-Chickens,
pubmed-meshheading:7794167-Concanavalin A,
pubmed-meshheading:7794167-Conjunctiva,
pubmed-meshheading:7794167-Female,
pubmed-meshheading:7794167-Head,
pubmed-meshheading:7794167-Lipopolysaccharides,
pubmed-meshheading:7794167-Lymphocyte Activation,
pubmed-meshheading:7794167-Lymphoid Tissue,
pubmed-meshheading:7794167-Male,
pubmed-meshheading:7794167-Salmonella typhimurium,
pubmed-meshheading:7794167-T-Lymphocytes
|
| pubmed:articleTitle |
Mitogenic responses of the head-associated lymphoid tissues of the chicken.
|
| pubmed:affiliation |
Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, Iowa 50011, USA.
|
| pubmed:publicationType |
Journal Article
|