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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0035820,
umls-concept:C0040648,
umls-concept:C0040679,
umls-concept:C0086860,
umls-concept:C0087140,
umls-concept:C0205070,
umls-concept:C0205103,
umls-concept:C0205263,
umls-concept:C0205369,
umls-concept:C0405581,
umls-concept:C0597170,
umls-concept:C0600508,
umls-concept:C1314939,
umls-concept:C1521761,
umls-concept:C1817144,
umls-concept:C1879547
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pubmed:issue |
7
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pubmed:dateCreated |
1995-7-21
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pubmed:abstractText |
A mesenchymal-epithelial cell interaction exists in the testis between the Sertoli cells that form the seminiferous tubule and the mesenchymal-derived peritubular myoid cells that surround the tubule. Analysis of the mesenchymal-epithelial interactions between these cells revealed the local production of a mesenchymal factor, PModS. PModS modulates the differentiated functions of Sertoli cells in vitro, including stimulation of the iron-binding protein transferrin (Tf). Previous results have indicated that PModS-induced Tf gene expression involves the activation of immediate early genes. One of the immediate early genes was identified as c-fos. The importance of c-fos was demonstrated in the current study when a c-fos antisense oligonucleotide was found to inhibit the ability of PModS to induce the expression of a Tf promoter-chloramphenicol acetyltransferase (CAT) construct. The regulation of c-fos by PModS was investigated with various CAT constructs containing segments of the c-fos promoter, such as the serum response element (SRE), sis-inducible element (SIE), cAMP response element (CRE), and phorbol ester/TPA response element (TRE), transfected into cultured Sertoli cells. PModS has no effect on cAMP response element-CAT or TRE-CAT, suggesting that PModS does not act through stimulation of cAMP and protein kinase C pathways. PModS was found to activate the c-fos SRE-CAT construct and the SIE-CAT construct. A construct containing both SIE and SRE was stimulated to the same degree as either element alone. Gel mobility shift assays using nuclear extracts from PModS-stimulated Sertoli cells and a radiolabeled SRE oligonucleotide resulted in retarded mobility of a DNA-protein complex. A gel shift with a SRE oligonucleotide containing an ETS domain resulted in a unique shift only detected in PModS stimulated cells. PModS also promoted a gel shift with the SIE that is adjacent to the SRE on the c-fos promoter. The data imply that PModS can activate the c-fos promoter through the SRE and SIE. PModS caused a labeled activating protein 1 (AP1) oligonucleotide to form a DNA-protein complex, indicating activation of the c-fos gene and binding of the c-fos/jun complex. To study the downstream regulation of Sertoli cell differentiation, Tf gene expression was examined. CAT constructs containing deletion mutants of a 3-kilobase (kb) mouse Tf promoter were used. When transfected into Sertoli cells the 581-base pair Tf minimal promoter had only a slight response to PModS, but was activated by FSH. The 2.6-kb Tf promoter construct responded to PModS.(ABSTRACT TRUNCATED AT 400 WORDS)
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/P-Mod-S paracrine factor,
http://linkedlifedata.com/resource/pubmed/chemical/Testicular Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0013-7227
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
136
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pubmed:geneSymbol |
c-fos
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3046-53
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7789331-Animals,
pubmed-meshheading:7789331-Base Sequence,
pubmed-meshheading:7789331-Binding Sites,
pubmed-meshheading:7789331-Cell Differentiation,
pubmed-meshheading:7789331-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7789331-DNA,
pubmed-meshheading:7789331-Gene Expression,
pubmed-meshheading:7789331-Genes, fos,
pubmed-meshheading:7789331-Male,
pubmed-meshheading:7789331-Molecular Sequence Data,
pubmed-meshheading:7789331-Promoter Regions, Genetic,
pubmed-meshheading:7789331-Rats,
pubmed-meshheading:7789331-Sertoli Cells,
pubmed-meshheading:7789331-Testicular Hormones,
pubmed-meshheading:7789331-Transcription Factors,
pubmed-meshheading:7789331-Transferrin
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pubmed:year |
1995
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pubmed:articleTitle |
Role of specific response elements of the c-fos promoter and involvement of intermediate transcription factor(s) in the induction of Sertoli cell differentiation (transferrin promoter activation) by the testicular paracrine factor PModS.
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pubmed:affiliation |
Reproductive Endocrinology Center, University of California, San Francisco 94143-0556, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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