pubmed-article:7789327 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0007137 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0070099 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
pubmed-article:7789327 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
pubmed-article:7789327 | pubmed:issue | 7 | lld:pubmed |
pubmed-article:7789327 | pubmed:dateCreated | 1995-7-21 | lld:pubmed |
pubmed-article:7789327 | pubmed:abstractText | PTH and PTH-related peptides (PTHrPs) interact with a common PTH/PTHrP receptor (type I), which is expressed in many tissues, including bone and kidney. Amino-terminal PTH and PTHrPs also recognize receptors in several nonclassical PTH target tissues, and in some of these, the signaling mechanisms differ qualitatively from those of the classical type I receptor. In normal keratinocytes and squamous carcinoma cell lines, PTH and PTHrP stimulate a rise in intracellular calcium, but not cAMP, suggesting the existence of an alternate, type II PTH/PTHrP receptor. SqCC/Y1 squamous carcinoma cells stably expressing the type I receptor displayed sensitive intracellular cAMP responses to PTHrP and PTH, indicating that these cells express functional GS proteins and that the type I receptor is capable of signaling through adenylyl cyclase in this cell line. Therefore, the endogenous type II receptor in SqCC/Y1 cells differs from the cloned type I receptor. We next examined whether messenger RNA (mRNA) from keratinocytes and squamous cell lines could hybridize to a human type I PTH/PTHrP receptor complementary DNA [1.9 kilobases (kb)]. No type I receptor mRNA (2.3 kb) was detected in polyadenylated RNA from any of the squamous cell lines. However, squamous cell lines did express several mRNA transcripts that hybridized with the type I receptor probe, yet were smaller (1 and 1.5 kb) or larger (3.5-5 kb) than the cloned receptor mRNA. The predominant mRNA in two squamous carcinoma cell lines and normal keratinocytes was a 1-kb transcript. Northern analysis with five different region-specific probes that span the entire coding region of the human type I receptor was used to map homologous regions within each of the transcripts. Several of the transcripts identified in squamous lines are also present in polyadenylated RNA from SaOS-2 human bone cells, but a unique 1-kb transcript hybridizing to probe 2 (nucleotides 490-870) was observed only in squamous cells. The smaller 1- and 1.5-kb transcripts did not hybridize to probes corresponding to the extreme 5'- and 3'-coding regions of the type I receptor complementary DNA. Ribonuclease protection analysis employing riboprobes that correspond to the five region-specific DNA probes revealed strong RNA signals of the expected size in SaOS-2 cells, but no hybridization with squamous cell RNA. Several smaller, but minor, bands that were unique to squamous cells were observed with riboprobe 2 only, suggesting partial homology of this region with the type I receptor.(ABSTRACT TRUNCATED AT 400 WORDS) | lld:pubmed |
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pubmed-article:7789327 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:7789327 | pubmed:language | eng | lld:pubmed |
pubmed-article:7789327 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7789327 | pubmed:citationSubset | AIM | lld:pubmed |
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pubmed-article:7789327 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:7789327 | pubmed:month | Jul | lld:pubmed |
pubmed-article:7789327 | pubmed:issn | 0013-7227 | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:JüppnerHH | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:KatzPP | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:SegreG VGV | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:VasavadaR CRC | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:Abou-SamraA... | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:SchipaniEE | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:UrenaPP | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:OrloffJ JJJ | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:BehalAA | lld:pubmed |
pubmed-article:7789327 | pubmed:author | pubmed-author:PhilbrickW... | lld:pubmed |
pubmed-article:7789327 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:7789327 | pubmed:volume | 136 | lld:pubmed |
pubmed-article:7789327 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:7789327 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:7789327 | pubmed:pagination | 3016-23 | lld:pubmed |
pubmed-article:7789327 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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pubmed-article:7789327 | pubmed:meshHeading | pubmed-meshheading:7789327-... | lld:pubmed |
pubmed-article:7789327 | pubmed:year | 1995 | lld:pubmed |
pubmed-article:7789327 | pubmed:articleTitle | Further evidence for a novel receptor for amino-terminal parathyroid hormone-related protein on keratinocytes and squamous carcinoma cell lines. | lld:pubmed |
pubmed-article:7789327 | pubmed:affiliation | Division of Endocrinology and Metabolism, West Haven Veterans Affairs Medical Center, Connecticut 06516, USA. | lld:pubmed |
pubmed-article:7789327 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:7789327 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:7789327 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
pubmed-article:7789327 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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