Linked Life Data

7788960

Source:http://linkedlifedata.com/resource/pubmed/id/7788960

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-7-27
pubmed:abstractText
A sensitive method of continuous on-line radio-high performance liquid chromatography (HPLC) was used to detect the specific radio-labelled acyl-carnitine esters derived from the oxidation of [U-14C]3-methyl-2-oxopentanoate by rat liver and muscle mitochondrial fractions. The recoveries of carnitine, acetyl-carnitine, propionyl-carnitine, 2-methylbutyryl-carnitine, and hexanoyl-carnitine were 98.7% (+/- 5.4; SEM, n = 3), 91.4% (+/- 7.6), 89.4% (+/- 5.2), 84.6% (+/- 6.8), and 87.9% (+/- 7.8), respectively, from quenched mitochondrial incubations. This method demonstrated that rat liver and muscle mitochondria generate acetyl-carnitine, propionyl-carnitine and 2-methylbutyryl-carnitine when incubated with [U-14C]3-methyl-2-oxopentanoate in the presence of carnitine. The production of acetyl-carnitine was almost similar in the 2 tissues. Muscle mitochondria produced higher amounts of propionyl-carnitine and 2-methylbutyryl-carnitine than liver mitochondria. These observations suggest a limited utilization of propionyl-CoA by muscle mitochondria which, through a mechanism of feed-back inhibition, may have contributed to the accumulation of 2-methylbutyryl-CoA. This study provides further evidence for the importance of carnitine in the modulation of the mitochondrial [acyl-CoA/[CoA] pool.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0147-958X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
144-51
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Production of acyl-carnitines from the metabolism of [U-14C]3-methyl-2-oxopentanoate by rat liver and skeletal muscle mitochondria.
pubmed:affiliation
Department of Child Health, Medical School, University of Newcastle Upon Tyne, U.K.
pubmed:publicationType
Journal Article