Linked Life Data

7788849

Source:http://linkedlifedata.com/resource/pubmed/id/7788849

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-7-26
pubmed:databankReference
pubmed:abstractText
The broad spectrum of biological responses associated with exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) is believed to be due to the alteration in expression of TCDD-inducible genes. The aim of this study was to investigate the effects of TCDD on the in vivo tissue-specific expression of the recently identified TCDD-inducible cytochrome P450 CYP1B1 [Sutter et al. (1994) J. Biol. Chem., 269, 13092-13099] in Sprague-Dawley rats. We cloned the 5.0 kb rat homolog of CYP1B1 from a TCDD-treated rat liver cDNA library and showed that the rat and human CYP1B1 predicted amino acid sequences are 80% identical. RNA hybridization analysis showed that CYP1B1 is constitutively expressed in the adrenal glands and also in the testes of untreated rats. This tissue distribution suggests that CYP1B1 may be a physiological steroid hydroxylase. Seventy-two hours post-administration of 25 micrograms/kg body wt TCDD by gavage, steady-state levels of the 5.1 kb CYP1B1 RNA were increased > 50-fold in liver, and to a lesser extent in kidneys, lung, heart and ovaries. Average CYP1B1 RNA levels were significantly higher in the kidneys and livers of TCDD-treated females than in those from similarly treated males. In contrast, no significant sex-difference was observed in the levels of CYP1A1 in these tissues in TCDD-treated animals. In Sprague-Dawley rats, TCDD is a more potent hepatocarcinogen in females than in males. The induction of CYP1B1 in TCDD rat liver may be a contributing factor to the carcinogenic action of this persistent environmental pollutant.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:geneSymbol
CYP1A2, CYP1B1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1319-27
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7788849-Adrenal Glands, pubmed-meshheading:7788849-Amino Acid Sequence, pubmed-meshheading:7788849-Animals, pubmed-meshheading:7788849-Aryl Hydrocarbon Hydroxylases, pubmed-meshheading:7788849-Base Sequence, pubmed-meshheading:7788849-Cloning, Molecular, pubmed-meshheading:7788849-Cytochrome P-450 Enzyme System, pubmed-meshheading:7788849-Enzyme Induction, pubmed-meshheading:7788849-Female, pubmed-meshheading:7788849-Gene Expression, pubmed-meshheading:7788849-Genes, pubmed-meshheading:7788849-Liver, pubmed-meshheading:7788849-Male, pubmed-meshheading:7788849-Molecular Sequence Data, pubmed-meshheading:7788849-RNA, Messenger, pubmed-meshheading:7788849-Rats, pubmed-meshheading:7788849-Rats, Sprague-Dawley, pubmed-meshheading:7788849-Restriction Mapping, pubmed-meshheading:7788849-Sequence Alignment, pubmed-meshheading:7788849-Sequence Homology, Amino Acid, pubmed-meshheading:7788849-Sex Factors, pubmed-meshheading:7788849-Testis, pubmed-meshheading:7788849-Tetrachlorodibenzodioxin, pubmed-meshheading:7788849-Tissue Distribution
pubmed:year
1995
pubmed:articleTitle
Rat CYP1B1: an adrenal cytochrome P450 that exhibits sex-dependent expression in livers and kidneys of TCDD-treated animals.
pubmed:affiliation
Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't