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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-7-24
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pubmed:databankReference |
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pubmed:abstractText |
Kinectin is a kinesin-binding protein (Toyoshima et al., 1992) that is required for kinesin-based motility (Kumar et al., 1995). A kinectin cDNA clone containing a 4.7-kilobase insert was isolated from an embryonic chick brain cDNA library by immunoscreening with a panel of monoclonal antibodies. The cDNA contained an open reading frame of 1364 amino acids encoding a protein of 156 kDa. A bacterially expressed product of the full length cDNA bound purified kinesin. Transient expression in CV-1 cells gave an endoplasmic reticulum distribution that depended upon the N-terminal domain. Analysis of the predicted amino acid sequence indicated a highly hydrophobic near N-terminal stretch of 28 amino acids and a large portion (326-1248) of predicted alpha helical coiled coils. The 30-kDa fragment containing the N-terminal hydrophobic region was produced by cell-free in vitro translation and found to assemble with canine pancreas rough microsomes. Cleavage of the N terminus was not observed confirming its role as a potential transmembrane domain. Thus, the kinectin cDNA encodes a cytoplasmic-oriented integral membrane protein that binds kinesin and is likely to be a coiled-coil dimer.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1400364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1512292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1832505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1835460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1939050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-1946431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2031185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2041810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2042975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2138513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2142876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2178778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2314296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2443518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2478561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2524275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2537834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2537835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2743999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2807624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2974459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-2991600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3097028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3141429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3237683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3340536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3550801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-3714490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-6088076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-7108955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-7678851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-7905879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-8248779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-8449993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-8496962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787244-8514798
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7787244-Humans,
pubmed-meshheading:7787244-Animals,
pubmed-meshheading:7787244-Kidney,
pubmed-meshheading:7787244-Brain,
pubmed-meshheading:7787244-Chick Embryo,
pubmed-meshheading:7787244-Membrane Proteins,
pubmed-meshheading:7787244-Cell Membrane,
pubmed-meshheading:7787244-Base Sequence,
pubmed-meshheading:7787244-Protein Biosynthesis,
pubmed-meshheading:7787244-Endoplasmic Reticulum,
pubmed-meshheading:7787244-Amino Acid Sequence,
pubmed-meshheading:7787244-Models, Structural,
pubmed-meshheading:7787244-Cell Line,
pubmed-meshheading:7787244-Molecular Sequence Data,
pubmed-meshheading:7787244-Transcription, Genetic,
pubmed-meshheading:7787244-Cercopithecus aethiops,
pubmed-meshheading:7787244-Protein Structure, Secondary,
pubmed-meshheading:7787244-Receptors, Cell Surface,
pubmed-meshheading:7787244-Sequence Homology, Amino Acid,
pubmed-meshheading:7787244-Chromatography, Affinity,
pubmed-meshheading:7787244-Signal Transduction
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