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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-7-24
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pubmed:databankReference |
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pubmed:abstractText |
We have identified a human cDNA that is homologous to the chicken kinectin, a putative receptor for the organelle motor kinesin. The human cDNA clone hybridized to a single 4.6-kb mRNA species that codes for a protein of 156 kDa molecular mass. The predicted primary translation product contains an N-terminal transmembrane helix followed by a bipartite nuclear localization sequence and two further C-terminal leucine zipper motifs. In addition, the aminoacid sequence revealed a large region (327-1362) of predicted alpha-helical coiled coils. A monoclonal antibody CT-1 raised against a GST-kinectin fusion protein produced a perinuclear, endoplasmic reticulum-like staining pattern in diverse cell types from different species, indicating evolutionary conservation. Monoclonal antibody CT-1 and anti-chicken kinectin antibodies cross-reacted both in Western blotting and immunoprecipitation with a 160-kDa protein, confirming the antigenic identity of this 160-kDa protein with chicken kinectin. Epitope tagging studies revealed that the nuclear localization sequence motif of kinectin is not functional. Furthermore, a truncated kinesin cDNA lacking the N-terminal hydrophobic domain revealed a nonspecific cytoplasmic staining pattern. Together the data suggest that kinectin is an integral membrane protein anchored in the endoplasmic reticulum via a transmembrane domain.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-1512292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-1698283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-1991323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2031185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2041810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2042975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2522351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2688707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-2831066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-3141429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-3416198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-6090948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-6546418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-7276066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-7787244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-8341676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7787243-8388385
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/KTN1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
161-70
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7787243-Amino Acid Sequence,
pubmed-meshheading:7787243-Animals,
pubmed-meshheading:7787243-Antibodies, Monoclonal,
pubmed-meshheading:7787243-Base Sequence,
pubmed-meshheading:7787243-Biological Evolution,
pubmed-meshheading:7787243-Cell Nucleus,
pubmed-meshheading:7787243-Chickens,
pubmed-meshheading:7787243-Cloning, Molecular,
pubmed-meshheading:7787243-Conserved Sequence,
pubmed-meshheading:7787243-DNA, Complementary,
pubmed-meshheading:7787243-Humans,
pubmed-meshheading:7787243-Kinesin,
pubmed-meshheading:7787243-Leucine Zippers,
pubmed-meshheading:7787243-Membrane Proteins,
pubmed-meshheading:7787243-Molecular Sequence Data,
pubmed-meshheading:7787243-Protein Biosynthesis,
pubmed-meshheading:7787243-Receptors, Cell Surface,
pubmed-meshheading:7787243-Recombinant Proteins,
pubmed-meshheading:7787243-Sequence Homology, Amino Acid,
pubmed-meshheading:7787243-Subcellular Fractions
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pubmed:year |
1995
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pubmed:articleTitle |
Molecular cloning and characterization of human kinectin.
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pubmed:affiliation |
Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität München, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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