Linked Life Data

7785463

Source:http://linkedlifedata.com/resource/pubmed/id/7785463

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-7-20
pubmed:abstractText
We previously reported that bone marrow stromal cells produce insulin-like growth factors (IGF-I and -II), and that medium conditioned by marrow stromal cells stimulates osteoblast proliferation in vitro. The present study employed the rat tail-suspension model to unload the hindlimbs. It was designed to test the hypothesis that the development of osteopenia or osteoporosis could be due to a deficit in the osteogenic function of marrow stromal cells. Although tail suspension suppressed body weight during the first 3 days of an 11-day pair-fed study, the overall weight gain recorded by these animals was normal. Nevertheless, bone growth was inhibited by suspension. Similarly, the total adherent marrow stromal cell population harvested from the femurs and tibias was decreased by tail suspension, and only half the normal number of fibroblastic stromal cell colonies grew when they were cultured. The proliferation of alkaline-phosphatase-positive cells in the stroma was also inhibited. Northern hybridization revealed that the messenger RNA level for transforming growth factor-beta 2 and IGF-II in stromal cell was reduced by tail suspension. The production of IGF-II by marrow stromal cells was also decreased. The steady-state level of five different transcript sizes of IGF-I mRNA was altered differentially by tail suspension. Osteopontin mRNA was also reduced in marrow stromal cells from tail-suspended rats compared with the normal rats. These data suggest that skeletal unloading not only alters the mRNA level for growth factors and peptide production, but also affects the proliferation and osteogenic differentiation of marrow stromal cells. These changes may be responsible for the reduced bone formation in osteopenia and osteoporosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7785463-Animals, pubmed-meshheading:7785463-Bone Diseases, Metabolic, pubmed-meshheading:7785463-Bone Marrow Cells, pubmed-meshheading:7785463-Cell Adhesion, pubmed-meshheading:7785463-Cell Differentiation, pubmed-meshheading:7785463-Cell Division, pubmed-meshheading:7785463-Cells, Cultured, pubmed-meshheading:7785463-Collagen, pubmed-meshheading:7785463-Femur, pubmed-meshheading:7785463-Gene Expression Regulation, Developmental, pubmed-meshheading:7785463-Male, pubmed-meshheading:7785463-Osteoblasts, pubmed-meshheading:7785463-Osteogenesis, pubmed-meshheading:7785463-Osteopontin, pubmed-meshheading:7785463-Osteoporosis, pubmed-meshheading:7785463-RNA, Messenger, pubmed-meshheading:7785463-Random Allocation, pubmed-meshheading:7785463-Rats, pubmed-meshheading:7785463-Rats, Wistar, pubmed-meshheading:7785463-Sialoglycoproteins, pubmed-meshheading:7785463-Somatomedins, pubmed-meshheading:7785463-Stromal Cells, pubmed-meshheading:7785463-Tail, pubmed-meshheading:7785463-Tibia, pubmed-meshheading:7785463-Transforming Growth Factor beta, pubmed-meshheading:7785463-Weight-Bearing
pubmed:year
1995
pubmed:articleTitle
Rat tail suspension reduces messenger RNA level for growth factors and osteopontin and decreases the osteoblastic differentiation of bone marrow stromal cells.
pubmed:affiliation
Department of Orthopedics and Rehabilitation, University of Texas Medical Branch at Galveston, USA.
pubmed:publicationType
Journal Article, Comparative Study