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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-7-14
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pubmed:abstractText |
Transforming growth factor beta 1 (TGF-beta 1) is known to inhibit epithelial cell growth by inducing a G1 cell cycle arrest. We have studied the effect of TGF-beta 1 on protein binding to a transcription factor E2F consensus element in extracts from early passage human keratinocytes (HFKs) and a permanent human keratinocyte cell line (HaCaT). Treatment of these cells with TGF-beta 1 resulted in the formation of a DNA binding complex between the pRb-related protein p130 and E2F. Formation of the E2F-p130 complex correlated with inhibition of cell cycle progression in G1 and suppression of the E2F-regulated cdc2 gene. While p130 mRNA and protein levels were not influenced by TGF-beta 1, the activity of cyclin-dependent kinase 2 (Cdk2) towards p130 in vitro was inhibited. The results identify p130 as a downstream target of TGF-beta 1 and a possible mediator of the G1 cell cycle arrest.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RBL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Like Protein p130,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
10
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pubmed:geneSymbol |
c-myc,
cdc2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2079-84
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7784052-CDC2 Protein Kinase,
pubmed-meshheading:7784052-CDC2-CDC28 Kinases,
pubmed-meshheading:7784052-Cell Cycle,
pubmed-meshheading:7784052-Cells, Cultured,
pubmed-meshheading:7784052-Cyclin-Dependent Kinase 2,
pubmed-meshheading:7784052-Cyclin-Dependent Kinases,
pubmed-meshheading:7784052-Genes, myc,
pubmed-meshheading:7784052-Humans,
pubmed-meshheading:7784052-Keratinocytes,
pubmed-meshheading:7784052-Phosphoproteins,
pubmed-meshheading:7784052-Phosphorylation,
pubmed-meshheading:7784052-Protein Binding,
pubmed-meshheading:7784052-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7784052-Proteins,
pubmed-meshheading:7784052-Retinoblastoma-Like Protein p130,
pubmed-meshheading:7784052-Transforming Growth Factor beta
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pubmed:year |
1995
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pubmed:articleTitle |
The pRb-related protein p130 is a possible effector of transforming growth factor beta 1 induced cell cycle arrest in keratinocytes.
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pubmed:affiliation |
Institut für Klinische Molekularbiologie und Tumorgenetik, GSF-Forschungszentrum für Umwelt und Gesundheit, München, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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