Linked Life Data

778276

Source:http://linkedlifedata.com/resource/pubmed/id/778276

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1976-8-23
pubmed:abstractText
A micro-method was developed that allowed the proliferation of blood lymphocytes from individual mice incubated with phytohemagglutinin to be measured on repeated occasions. Lymphocytes were isolated from 0.4 ml of heparinized blood by centrifugation through a Ficoll-Hypaque solution. This amount of blood could be taken repeatedly from individual mice and yielded 4-8 X 10(5) lymphocytes. Fifty thousand lymphocytes were incubated with 0.2 ml of medium RPMI 1640 containing 5% human serum in round bottom micro-titer wells. Using this technique lymphocytes from individual mice of the same strain were shown to differ consistently in their responses to PHA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-105
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
A method to study in vitro proliferation of lymphocytes from individual mice on repeated occasions.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.