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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-7-20
|
| pubmed:abstractText |
Although a disturbed immune response to constituents of the gut mucosa has been implicated in the pathogenesis of inflammatory bowel disease, the mechanisms are still unclear. Intestinal T-cells derived from gut biopsies were propagated in vitro as single and co-cultures under different experimental conditions prior to flow cytometry. Intestinal T-cell lines from inflamed mucosa (n = 69) showed a significant (P < 0.001) decrease in CD4+ T-cells compared to T-cells from normal (n = 49) and uninflamed (n = 29) tissue specimens. Co-culturing of inflamed and uninflamed mucosa led to a normalization of CD4+ T-cells in cultures derived from inflamed mucosa. Analysis of supernatants revealed a significantly (P < 0.001) increased secretion of IL-4 under co-culture conditions. Moreover, stimulation of cultures derived from inflamed mucosa with rIL-4 led to a significant (P < 0.001) increase in CD4+ T-cells, whereas anti-IL-4 antibodies or IFN-gamma supplementation of T-cells derived from uninflamed mucosa significantly (P < 0.001) reduced the CD4+ subset. Treatment with IFN-gamma and anti-IL-4 antibodies did not affect the phenotype of T-cells derived from inflamed mucosa. These data suggest that IL-4 might play a key role in the intestinal immune response. Furthermore, this in vitro system allows the investigation of mucosal immune mechanisms in more detail under standardized conditions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0014-2972
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
155-64
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7781661-Adolescent,
pubmed-meshheading:7781661-Adult,
pubmed-meshheading:7781661-Aged,
pubmed-meshheading:7781661-Antibodies, Monoclonal,
pubmed-meshheading:7781661-Antigens, CD,
pubmed-meshheading:7781661-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7781661-Cells, Cultured,
pubmed-meshheading:7781661-Colitis, Ulcerative,
pubmed-meshheading:7781661-Crohn Disease,
pubmed-meshheading:7781661-Cytokines,
pubmed-meshheading:7781661-Female,
pubmed-meshheading:7781661-Flow Cytometry,
pubmed-meshheading:7781661-Humans,
pubmed-meshheading:7781661-Immunophenotyping,
pubmed-meshheading:7781661-Inflammation,
pubmed-meshheading:7781661-Inflammatory Bowel Diseases,
pubmed-meshheading:7781661-Intestinal Mucosa,
pubmed-meshheading:7781661-Male,
pubmed-meshheading:7781661-Middle Aged,
pubmed-meshheading:7781661-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:7781661-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:7781661-Reference Values,
pubmed-meshheading:7781661-T-Lymphocytes
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Phenotypic and immunoregulatory analysis of intestinal T-cells in patients with inflammatory bowel disease: evaluation of an in vitro model.
|
| pubmed:affiliation |
Abteilung Innere Medizin II, Gastroenterologie, Klinikum der Albert-Ludwigs-Universität, Freiburg, Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|