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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1995-7-19
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pubmed:databankReference |
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pubmed:abstractText |
Potassium uptake by guard cells represents part of the osmotic motor which drives stomatal opening. Patch-clamp measurements have identified inward rectifying K+ channels capable of mediating K+ uptake in guard cells and various other plant cell types. Here we report the molecular cloning and characterization of a voltage-dependent K+ channel (KST1) from potato (Solanum tuberosum L.) guard cells. In situ hybridization shows expression of kst1 in guard cells. Two-electrode voltage-clamp and patch-clamp studies of the gene product after cRNA injection into Xenopus oocytes identified KST1 as a slowly activating, voltage-dependent, inward rectifying K+ channel. The single channel current voltage curve was linear in the range -160 to +20 mV, with a deduced single channel conductance of 7 pS in symmetrical 100 mM K+. This channel type, modulated by pH changes within the physiological range, required ATP for activation. In line with the properties of a K(+)-selective channel, KST1 was permeable to K+, Rb+ and NH4+ and excluded Na+ and Li+. Cs+ at submillimolar concentrations blocked the channel in a voltage-dependent manner. Related studies on potato guard cell protoplasts confirmed the biophysical characteristics of the kst1 gene product (KST1) in the heterologous expression system. Therefore, KST1 represents a major K+ uptake channel in potato guard cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1419000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1528115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1534573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1562183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1570292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1585180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1701140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-1715403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-2126155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-2421601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-2435540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-2441470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-3039867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-3221838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-7532027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-7680431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-7680768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-7704050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-8065452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-8170937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-8450880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7781596-8966547
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
14
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pubmed:geneSymbol |
kst1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2409-16
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:7781596-Amino Acid Sequence,
pubmed-meshheading:7781596-Animals,
pubmed-meshheading:7781596-Base Sequence,
pubmed-meshheading:7781596-Cations,
pubmed-meshheading:7781596-Cloning, Molecular,
pubmed-meshheading:7781596-DNA, Complementary,
pubmed-meshheading:7781596-DNA, Plant,
pubmed-meshheading:7781596-Electrophysiology,
pubmed-meshheading:7781596-Female,
pubmed-meshheading:7781596-Gene Expression,
pubmed-meshheading:7781596-Genes, Plant,
pubmed-meshheading:7781596-Hydrogen-Ion Concentration,
pubmed-meshheading:7781596-Molecular Sequence Data,
pubmed-meshheading:7781596-Oocytes,
pubmed-meshheading:7781596-Plants,
pubmed-meshheading:7781596-Potassium Channels,
pubmed-meshheading:7781596-Sequence Homology, Amino Acid,
pubmed-meshheading:7781596-Solanum tuberosum,
pubmed-meshheading:7781596-Xenopus
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and electrophysiological analysis of KST1, an inward rectifying K+ channel expressed in potato guard cells.
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pubmed:affiliation |
Institut für Genbiologische Forschung Berlin GmbH, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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