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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-7-19
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pubmed:abstractText |
In Chlamydomonas reinhardtii, cytochrome c6 (cyt c6) is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized. In previous work, the copper-responsive regulation of cyt c6 synthesis was demonstrated to occur by control of transcription, with no contribution from post-transcriptional processes. To understand the mechanism underlying its regulation, the genomic DNA encoding cyt c6 (Cyc6) was analyzed for the presence of copper-responsive elements. Sequences lying between positions -127 and -7 with respect to the start site of transcription were found to be sufficient to confer copper-responsive expression on either a promoterless or a minimal beta-tubulin promoter-driven (arylsulfatase-encoding) reporter gene. Analysis of this 120-bp fragment indicated that copper-responsive elements lie in two distinct regions (between -110 to -56 and -127 to -109). ATG fusions between copper-insensitive promoters and the coding plus 3' untranslated region of the Cyc6 gene resulted in the accumulation of cyt c6 in copper-supplemented medium; this confirms earlier studies indicating a lack of post-transcriptional control in this copper-responsive pathway. In the context of a constitutive promoter (derived from the beta-tubulin gene), each region was found to function as an activator of transcription in copper-deficient cells, and the metal specificity of the response of reporter genes containing either one or both regions was identical to that of the endogenous Cyc6 gene. The copper-responsive synthesis of cyt c6 is thus attributed to these two 5' upstream sequences.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1314811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1320727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1326543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1561077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1570306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1620590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-16652963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1714451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-1863287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-202500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-208838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2105499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2156853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2293243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2539381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2583083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2592399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2672439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-2798100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3023330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3023849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3036842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3052856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3121586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3194392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3323819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-3339089,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-6326095,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8035797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8038605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8061521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8090746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8216201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8293472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8343519,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8370529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7780310-8407939
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-8
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:7780310-Animals,
pubmed-meshheading:7780310-Arylsulfatases,
pubmed-meshheading:7780310-Base Sequence,
pubmed-meshheading:7780310-Binding Sites,
pubmed-meshheading:7780310-Cells, Cultured,
pubmed-meshheading:7780310-Chlamydomonas reinhardtii,
pubmed-meshheading:7780310-Copper,
pubmed-meshheading:7780310-Cytochrome b Group,
pubmed-meshheading:7780310-Cytochrome b6f Complex,
pubmed-meshheading:7780310-DNA Mutational Analysis,
pubmed-meshheading:7780310-Gene Expression Regulation, Plant,
pubmed-meshheading:7780310-Genes, Plant,
pubmed-meshheading:7780310-Genes, Reporter,
pubmed-meshheading:7780310-Molecular Sequence Data,
pubmed-meshheading:7780310-Promoter Regions, Genetic,
pubmed-meshheading:7780310-Protein Binding,
pubmed-meshheading:7780310-Sequence Deletion,
pubmed-meshheading:7780310-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7780310-Transcription, Genetic,
pubmed-meshheading:7780310-Transcription Factors,
pubmed-meshheading:7780310-Transformation, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
Two copper-responsive elements associated with the Chlamydomonas Cyc6 gene function as targets for transcriptional activators.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of California at Los Angeles 90095-1569, USA.
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