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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-7-18
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pubmed:abstractText |
A cancer cell transfection model was used to evaluate biological activity of soluble IL-6 receptor (sIL-6R) in vivo. B-78 melanoma cells were stably transfected with cDNAs encoding human IL-6, murine sIL-6R and human leukaemia inhibitory factor (LIF). Control and transfected cells were intravenously (i.v.) and/or subcutaneously (s.c.) injected into B57BL/6 x C3H or SCID mice. Whereas B-78 cells formed tumours and lung metastasis in injected animals, transfected animals, transfected cells showed greatly reduced tumour and metastasis formation. Transfection of IL-6, sIL-6R or LIF had similar protective effects. The combination of IL-6 and sIL-6R was most effective. Kinetic analysis demonstrated a 3 week lag period between formation of tumours by B-78 cells and the combination of B-78 cells transfected with IL-6 and sIL-6R. No such lag phase was seen when B-78-IL-6 or B-78-IL-6 or B-78-sIL-6R were injected alone. These results indicate that IL-6 alone exhibits a different quality of activity when compared to the IL-6-soluble receptor complex. Our results demonstrate for the first time that sIL-6R is a biologically active molecule in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
142-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7780033-Animals,
pubmed-meshheading:7780033-Blotting, Northern,
pubmed-meshheading:7780033-Cell Division,
pubmed-meshheading:7780033-Cell Line,
pubmed-meshheading:7780033-Cytosol,
pubmed-meshheading:7780033-DNA, Complementary,
pubmed-meshheading:7780033-Gene Expression,
pubmed-meshheading:7780033-Glycoproteins,
pubmed-meshheading:7780033-Humans,
pubmed-meshheading:7780033-Intercellular Adhesion Molecule-1,
pubmed-meshheading:7780033-Interleukin-6,
pubmed-meshheading:7780033-Kinetics,
pubmed-meshheading:7780033-Lung Neoplasms,
pubmed-meshheading:7780033-Melanoma, Experimental,
pubmed-meshheading:7780033-Mice,
pubmed-meshheading:7780033-Mice, Inbred C3H,
pubmed-meshheading:7780033-Mice, Inbred C57BL,
pubmed-meshheading:7780033-Mice, SCID,
pubmed-meshheading:7780033-Neoplasm Metastasis,
pubmed-meshheading:7780033-Protein Biosynthesis,
pubmed-meshheading:7780033-Receptors, Interleukin,
pubmed-meshheading:7780033-Receptors, Interleukin-6,
pubmed-meshheading:7780033-Recombinant Proteins,
pubmed-meshheading:7780033-Tissue Inhibitor of Metalloproteinase-2,
pubmed-meshheading:7780033-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:7780033-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Soluble interleukin 6 receptor is biologically active in vivo.
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pubmed:affiliation |
Department of Cancer Immunology, Great Poland Cancer Center, Poznan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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