Linked Life Data

7779948

Source:http://linkedlifedata.com/resource/pubmed/id/7779948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-7-18
pubmed:abstractText
Endothelial cells were obtained from white-tailed deer carotid arteries and umbilical vessels by instilling a weak collagenase type II solution. Cell growth was best when cultures were grown in plates coated with either fibronectin or laminin. Several commercially available media supported the growth of these cells when supplemented with a commercially available endothelial growth supplement and 10% fetal calf serum. Cells obtained could be characterized as endothelium by ultrastructural characteristics and by the uptake of fluorescent acetylated low-density lipoprotein. Cells stained positive for factor VIII-related antigen up to passage 2, but staining was inconsistent by passage 3, and no immunoreactivity could be demonstrated after passage 4.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1040-6387
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Isolation and culture of large vessel endothelium from white-tailed deer (Odocoileus virginianus).
pubmed:affiliation
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't