Linked Life Data

7779399

Source:http://linkedlifedata.com/resource/pubmed/id/7779399

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-7-20
pubmed:abstractText
Historically, forensic and clinical laboratories utilize serological techniques to identify ABO blood types. These techniques rely on the detection of ABO-associated proteins and are sensitive with very accurate results. This laboratory has simplified the identification of ABO types by taking advantage of previously reported ABO DNA sequence differences. The Rapid-ABO technique involves a two-step process: (i) amplification of DNA samples using primer sets specific for the ABO alleles and (ii) electrophoresis and visualization of amplified ABO fragments on a 3% MetaPhor agarose gel. The major advantage of the Rapid-ABO technique is the identification of ABO genotypes compared to serological tests for ABO phenotypes. This two-step process identifies six possible ABO genotypes including AB, AA, BB, AO, BO and OO. The Rapid-ABO protocol works well with DNA extracted organically or using Chelex 100. Results can be obtained in less than a day utilizing 2 ng of DNA in the amplification reaction. Analysis of 23 animal species shows the Rapid-ABO primers amplify ABO alleles from only human, chimpanzee and gorilla DNA.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
478-83
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Identification of ABO alleles on forensic-type specimens using rapid-ABO genotyping.
pubmed:affiliation
Palm Beach County Sheriff's Office Crime Laboratory, FL, USA.
pubmed:publicationType
Journal Article