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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1995-7-20
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pubmed:abstractText |
A recently developed mRNA differential display technique has a potential power for identifying genes that are differentially expressed in a variety of in vitro and in vivo systems. One critical feature of this technique is to display most of the mRNA population on a sequencing gel after polymerase chain reaction using a 5' decamer and a 3' T12MN anchored primer. However, these primers are too small to be successfully used as a sequencing primer using the classical sequencing protocol. In the present report we described the application of an extended primer set for the re-amplification after mRNA differential display, which renders the amplified DNA suitable for direct sequencing using either of these extended oligonucleotides as a primer without further subcloning. This improved technique will greatly save the time, cost and labor-intensive work for the discovery of genes using mRNA differential display.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0736-6205
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
448-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7779394-Animals,
pubmed-meshheading:7779394-Base Sequence,
pubmed-meshheading:7779394-Blotting, Northern,
pubmed-meshheading:7779394-Cells, Cultured,
pubmed-meshheading:7779394-DNA Primers,
pubmed-meshheading:7779394-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7779394-Molecular Sequence Data,
pubmed-meshheading:7779394-Polymerase Chain Reaction,
pubmed-meshheading:7779394-RNA, Messenger,
pubmed-meshheading:7779394-Rats,
pubmed-meshheading:7779394-Rats, Inbred Strains,
pubmed-meshheading:7779394-Rats, Inbred WKY,
pubmed-meshheading:7779394-Rats, Wistar,
pubmed-meshheading:7779394-Sequence Analysis, DNA,
pubmed-meshheading:7779394-Transcription, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
Direct sequencing of DNA isolated from mRNA differential display.
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pubmed:affiliation |
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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