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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-7-7
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pubmed:abstractText |
The human immunodeficiency virus-1 transactivator protein (tat) was codelivered efficiently with a reporter gene under the control of a tat-responsive DNA element using different formulations of cationic liposomes. Expression of a tat-responsive reporter gene was induced by incubating cells with a mixture of purified recombinant tat protein, reporter DNA, and liposomes. Different cell lines were tested successfully as targets for the codelivery. Tat was shown to trans-activate the codelivered virus promoter specifically in the cells tested. Codelivery of tat with DNA is a useful model for studying the function of trans-acting factors and their cis-acting DNA elements. The currently available methods such as foot-printing only reveal the binding, but not the functional consequence of the binding, of the factor with the element. In addition, this system may prove useful as a model for high level and regulated transgene expression in target cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-dielaidoylphosphatidylethanolami...,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
225
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-93
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7778790-Animals,
pubmed-meshheading:7778790-Base Sequence,
pubmed-meshheading:7778790-CHO Cells,
pubmed-meshheading:7778790-Carcinoma, Squamous Cell,
pubmed-meshheading:7778790-Cell Line,
pubmed-meshheading:7778790-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7778790-Cricetinae,
pubmed-meshheading:7778790-DNA Primers,
pubmed-meshheading:7778790-Drug Carriers,
pubmed-meshheading:7778790-Gene Products, tat,
pubmed-meshheading:7778790-HIV Long Terminal Repeat,
pubmed-meshheading:7778790-HIV-1,
pubmed-meshheading:7778790-HeLa Cells,
pubmed-meshheading:7778790-Humans,
pubmed-meshheading:7778790-Kidney,
pubmed-meshheading:7778790-Liposomes,
pubmed-meshheading:7778790-Molecular Sequence Data,
pubmed-meshheading:7778790-Mutagenesis,
pubmed-meshheading:7778790-Phosphatidylethanolamines,
pubmed-meshheading:7778790-Plasmids,
pubmed-meshheading:7778790-Polymerase Chain Reaction,
pubmed-meshheading:7778790-Recombinant Proteins,
pubmed-meshheading:7778790-Sequence Deletion,
pubmed-meshheading:7778790-Transcriptional Activation,
pubmed-meshheading:7778790-Transfection,
pubmed-meshheading:7778790-Tumor Cells, Cultured,
pubmed-meshheading:7778790-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1995
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pubmed:articleTitle |
Codelivery to mammalian cells of a transcriptional factor with cis-acting element using cationic liposomes.
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pubmed:affiliation |
Department of Pharmacology, University of Pittsburgh, School of Medicine, Pennsylvania 15261, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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