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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23-24
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pubmed:dateCreated |
1995-7-11
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pubmed:abstractText |
Binding and photoactivation of a cannabinoid-derived ligand to intact mouse peritoneal cells has resulted in the labelling of a G-protein gamma-subunit. The assignment of structure is based on the product's physical characteristics and its ability to react with a polyclonal antiserum raised against the partial amino acid sequence for a gamma-subunit expressed in spleen cells. The binding characteristics of the ligand to the cells suggests that this gamma-subunit, and its associated alpha and beta subunits, are located in close proximity to one of the transmembrane cannabinoid receptors. Our findings further suggest a possible experimental approach to identifying receptor-G-protein complexes in intact cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1991-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7776823-Amino Acid Sequence,
pubmed-meshheading:7776823-Animals,
pubmed-meshheading:7776823-Cells, Cultured,
pubmed-meshheading:7776823-Chromatography, High Pressure Liquid,
pubmed-meshheading:7776823-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7776823-Female,
pubmed-meshheading:7776823-GTP-Binding Proteins,
pubmed-meshheading:7776823-Ligands,
pubmed-meshheading:7776823-Mice,
pubmed-meshheading:7776823-Molecular Sequence Data,
pubmed-meshheading:7776823-Peritoneal Cavity,
pubmed-meshheading:7776823-Photochemistry,
pubmed-meshheading:7776823-Precipitin Tests,
pubmed-meshheading:7776823-Protein Binding,
pubmed-meshheading:7776823-Receptors, Cannabinoid,
pubmed-meshheading:7776823-Receptors, Drug,
pubmed-meshheading:7776823-Tetrahydrocannabinol
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pubmed:year |
1995
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pubmed:articleTitle |
Cannabinoid photolabelling of a G-protein gamma-subunit in mouse peritoneal cells.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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