Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-7-13
|
| pubmed:abstractText |
The mechanisms underlying the induction of immunological tolerance after feeding soluble exogenous antigens, including proteins and haptens, are still unclear. Using a model of oral tolerance to the contact-sensitizing hapten 2,4-dinitrochlorobenzene (DNCB), we have compared the ability-of intestinal epithelial cells and of Peyer's patch APC to present DNCB in vitro or ex vivo after oral feeding, to specific peripheral lymph node T cells from DNCB-sensitized mice. In contrast to Peyer's patch APC, which induce efficient hapten-specific T cell activation upon exposure to the hapten either in vitro or in vivo, mature MHC class-II-positive intestinal epithelial cells were unable to induce T cell activation in either case. Interestingly, enterocytes from DNCB-fed mice exerted a dramatic inhibitory effect on the proliferative response of hapten-primed T cells in response to dinitrobenzene sulfonate presented by syngeneic spleen cells. This inhibitory effect, which was also observed with supernatant of intestinal epithelial cells from DNCB-fed mice, could be reversed by neutralizing anti-transforming growth factor (TGF)-beta antibodies. In addition, pre-incubation of hapten-sensitized T cells with enterocytes from DNCB-fed mice induced T cell anergy, which could be reversed by exogenous interleukin-2 or interleukin-4. These data demonstrate that intestinal epithelial cells activated in vivo by oral administration of DNCB are able to block proliferation of activated T cells through secretion of immunosuppressive cytokines such as TGF-beta. It is proposed that intestinal epithelial cells may play a significant role in oral tolerance by limiting T cell-mediated hypersensitivity responses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,4-dinitrofluorobenzene sulfonic...,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrochlorobenzene,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrofluorobenzene,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1385-90
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7774642-Administration, Oral,
pubmed-meshheading:7774642-Animals,
pubmed-meshheading:7774642-Antigen-Presenting Cells,
pubmed-meshheading:7774642-Culture Media, Conditioned,
pubmed-meshheading:7774642-Dermatitis, Allergic Contact,
pubmed-meshheading:7774642-Dinitrochlorobenzene,
pubmed-meshheading:7774642-Dinitrofluorobenzene,
pubmed-meshheading:7774642-Epithelial Cells,
pubmed-meshheading:7774642-Female,
pubmed-meshheading:7774642-Haptens,
pubmed-meshheading:7774642-Histocompatibility Antigens Class II,
pubmed-meshheading:7774642-Immune Tolerance,
pubmed-meshheading:7774642-Immunization,
pubmed-meshheading:7774642-Interleukin-2,
pubmed-meshheading:7774642-Interleukin-4,
pubmed-meshheading:7774642-Intestinal Absorption,
pubmed-meshheading:7774642-Intestinal Mucosa,
pubmed-meshheading:7774642-Lymph Nodes,
pubmed-meshheading:7774642-Lymphocyte Activation,
pubmed-meshheading:7774642-Mice,
pubmed-meshheading:7774642-Mice, Inbred C3H,
pubmed-meshheading:7774642-Peyer's Patches,
pubmed-meshheading:7774642-Spleen,
pubmed-meshheading:7774642-T-Lymphocyte Subsets,
pubmed-meshheading:7774642-Transforming Growth Factor beta
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Oral tolerance to haptens: intestinal epithelial cells from 2,4-dinitrochlorobenzene-fed mice inhibit hapten-specific T cell activation in vitro.
|
| pubmed:affiliation |
INSERM U404, Immunité et Vaccination, Institut Pasteur de Lyon, France.
|
| pubmed:publicationType |
Journal Article
|