|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1995-7-13
|
|
pubmed:abstractText |
The solution structure of the specific complex between the high mobility group (HMG) domain of SRY (hSRY-HMG), the protein encoded by the human testis-determining gene, and its DNA target site in the promoter of the müllerian inhibitory substance gene has been determined by multidimensional NMR spectroscopy. hSRY-HMG has a twisted L shape that presents a concave surface (made up of three helices and the N- and C-terminal strands) to the DNA for sequence-specific recognition. Binding of hSRY-HMG to its specific target site occurs exclusively in the minor groove and induces a large conformational change in the DNA. The DNA in the complex has an overall 70 degrees-80 degrees bend and is helically unwound relative to classical A- and B-DNA. The structure of the complex reveals the origin of sequence-specific binding within the HMG-1/HMG-2 family and provides a framework for understanding the effects of point mutations that cause 46X,Y sex reversal at the atomic level.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Mullerian Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/SRY protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sex-Determining Region Y Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Testicular Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
2
|
|
pubmed:volume |
81
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
705-14
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7774012-Anti-Mullerian Hormone,
pubmed-meshheading:7774012-Binding Sites,
pubmed-meshheading:7774012-DNA-Binding Proteins,
pubmed-meshheading:7774012-Disorders of Sex Development,
pubmed-meshheading:7774012-Female,
pubmed-meshheading:7774012-Glycoproteins,
pubmed-meshheading:7774012-Growth Inhibitors,
pubmed-meshheading:7774012-Humans,
pubmed-meshheading:7774012-Karyotyping,
pubmed-meshheading:7774012-Magnetic Resonance Spectroscopy,
pubmed-meshheading:7774012-Male,
pubmed-meshheading:7774012-Models, Molecular,
pubmed-meshheading:7774012-Nuclear Proteins,
pubmed-meshheading:7774012-Oligonucleotides,
pubmed-meshheading:7774012-Point Mutation,
pubmed-meshheading:7774012-Protein Binding,
pubmed-meshheading:7774012-Sex Chromosome Aberrations,
pubmed-meshheading:7774012-Sex Differentiation,
pubmed-meshheading:7774012-Sex-Determining Region Y Protein,
pubmed-meshheading:7774012-Testicular Hormones,
pubmed-meshheading:7774012-Transcription, Genetic,
pubmed-meshheading:7774012-Transcription Factors,
pubmed-meshheading:7774012-X Chromosome,
pubmed-meshheading:7774012-Y Chromosome
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex.
|
|
pubmed:affiliation |
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
