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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-7-10
|
| pubmed:abstractText |
The characterisation of oligosaccharide fragments isolated from enzymatically depolymerised porcine intestinal heparin is required in order to probe structure/function relationships of heparin in anticoagulation, antiangiogenesis and antiviral activity. We have used both LSI-MS and 600-MHz 1H NMR with chemical shift assignment by comprehensive 1H-1H TOCSY experiments to fully characterise the major oligosaccharide components including 4 tetrasaccharides, 3 hexasaccharides, and 2 octasaccharides. One of the octasaccharides has not been identified previously and has the structure: delta UA(2S)-GlcNS(6S)-IdoA(2S)-GlcNS(6S)-IdoA(2S)- GlcNS(6S)-GlcA-GlcNS(6S), where delta UA is 4,5-unsaturated uronic acid (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid), GlcN is --> 4)-alpha-D-glucosamine, IdoA is --> 4)-alpha-L-iduronic acid, GlcA is --> 4)-beta-D-glucuronic acid, and 2-O-, 6-O-, and 2-N-sulfate are abbreviated to 2S, 6S, and NS, respectively. Nearly complete NMR proton chemical shifts are reported for this data set. In addition a novel approach involving oxymercuration-lipid conjugation was used to independently assign sulfate substitution on the delta UA residues.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0008-6215
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
3
|
| pubmed:volume |
269
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
139-56
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7773986-Animals,
pubmed-meshheading:7773986-Carbohydrate Sequence,
pubmed-meshheading:7773986-Chromatography, High Pressure Liquid,
pubmed-meshheading:7773986-Heparin,
pubmed-meshheading:7773986-Intestines,
pubmed-meshheading:7773986-Magnetic Resonance Spectroscopy,
pubmed-meshheading:7773986-Molecular Sequence Data,
pubmed-meshheading:7773986-Oligosaccharides,
pubmed-meshheading:7773986-Sequence Analysis,
pubmed-meshheading:7773986-Spectrometry, Mass, Secondary Ion,
pubmed-meshheading:7773986-Swine
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Characterisation by LSI-MS and 1H NMR spectroscopy of tetra-, hexa-, and octa-saccharides of porcine intestinal heparin.
|
| pubmed:affiliation |
Clinical Mass Spectrometry Section, MRC Clinical Research Centre, Harrow, Middx, United Kingdom.
|
| pubmed:publicationType |
Journal Article
|