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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-7-13
|
| pubmed:databankReference | |
| pubmed:abstractText |
The ferritin heavy (H) and middle (M) subunit cDNAs were isolated from the Atlantic salmon (Salmo salar) liver. Full-length clones encoding the ferritin M subunit of 176 residues were obtained by screening of a liver cDNA library. The evolutionary conserved iron-responsive element (IRE) was identified in the upstream untranslated region. Ferritin H cDNA was cloned by running reverse transcription-polymerase chain reaction (RT-PCR) on salmon liver mRNA. The salmon ferritin H subunit of 177 residues showed 67% sequence identity with the M subunit. Northern blot analysis revealed ferritin H mRNA in the liver, gonads, head kidney, heart, and spleen, whereas M subunit mRNA was found almost exclusively in the gonads. Polyclonal antibodies against both salmon ferritin H and M were raised in rabbits.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1053-6426
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
164-70
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7773334-Amino Acid Sequence,
pubmed-meshheading:7773334-Animals,
pubmed-meshheading:7773334-Antibody Formation,
pubmed-meshheading:7773334-Atlantic Ocean,
pubmed-meshheading:7773334-Base Sequence,
pubmed-meshheading:7773334-Blotting, Northern,
pubmed-meshheading:7773334-Cloning, Molecular,
pubmed-meshheading:7773334-DNA, Complementary,
pubmed-meshheading:7773334-Female,
pubmed-meshheading:7773334-Ferritins,
pubmed-meshheading:7773334-Liver,
pubmed-meshheading:7773334-Male,
pubmed-meshheading:7773334-Molecular Sequence Data,
pubmed-meshheading:7773334-Nucleic Acid Conformation,
pubmed-meshheading:7773334-Polymerase Chain Reaction,
pubmed-meshheading:7773334-Protein Conformation,
pubmed-meshheading:7773334-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7773334-Salmon,
pubmed-meshheading:7773334-Sequence Homology, Amino Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Two ferritin subunits of Atlantic salmon (Salmo salar): cloning of the liver cDNAs and antibody preparation.
|
| pubmed:affiliation |
Akvaforsk, Institute of Aquaculture Research, Aas, Norway.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|