Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-7-7
|
| pubmed:abstractText |
The minimum size DNA fragment (3011 bp) containing the entire phosphoenolpyruvate carboxylase [EC 4.1.1.31] gene of E. coli was cloned into a modified plasmid vector of high copy-number. The gene expression was directed by its own promoter and the content of the enzyme reached about 30% of total soluble protein of the transformed cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
B
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0916-8451
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:geneSymbol |
ppc
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
735-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7772842-Base Sequence,
pubmed-meshheading:7772842-Cloning, Molecular,
pubmed-meshheading:7772842-Escherichia coli,
pubmed-meshheading:7772842-Molecular Sequence Data,
pubmed-meshheading:7772842-Oligodeoxyribonucleotides,
pubmed-meshheading:7772842-Phosphoenolpyruvate Carboxylase,
pubmed-meshheading:7772842-Plasmids
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Construction of a plasmid for high level expression of Escherichia coli phosphoenolpyruvate carboxylase.
|
| pubmed:affiliation |
Department of Chemistry, Faculty of Science, Kyoto University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|