Linked Life Data

77708

Source:http://linkedlifedata.com/resource/pubmed/id/77708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1978-8-28
pubmed:abstractText
Golgi techniques, immunofluorescence for glial fibrillary acidic (GFA) protein, and electron microscopy (EM) were used to determine the nature of radial glia in the cerebrum of human fetuses ranging from 7 to 20 weeks of ovulation age. Successful Golgi impregnation of radial fibers was achieved in fetuses 12 weeks of age and older. These fibers spanned the entire thickness of the hemisphere. At the pial surface many of them branched and terminated in pyramidal end feet expansions. Indirect immunofluorescent preparations utilizing antiserum to GFA protein, a protein specific for astrocytes, demonstrated numerous radially oriented nearly parallel fluorescent fibres between the ventricular zone and pia mater. GFA protein-positive fibers were demonstrated in all fetal specimens examined with this technique (10 weeks of age and older). Along the outer border of the marginal zone they formed a horizontal GFA protein-containing subpial membrane. By EM there were numerous linear electron lucent astrocytic processes containing 8-9 nm filaments and occasional glycogen granules at all levels of the cerebrum. They were interspersed among smaller and darker neuronal processes containing 20-25 nm neurotubules, and were demonstrable at all fetal ages between 7 and 18 weeks. They formed pericapillary investments and subpial terminal expansions closely abutting basal lamina of pia mater in every specimen examined. On the basis of these combined analyses, we conclude that radial glial fibers in early human fetal cerebrum represent processes of immature astrocytes. Although subsequently undergoing further maturation, radial glia already possess fundamental immunocytochemical and morphological characteristics indicative of astrocytic differentiation. A significant implication of our findings is that the development of astrocytes in the human fetal brain occurs much earlier than formerly believed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-311
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Radial glia in the human fetal cerebrum: a combined Golgi, immunofluorescent and electron microscopic study.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.