Linked Life Data

7769002

Source:http://linkedlifedata.com/resource/pubmed/id/7769002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-7-6
pubmed:databankReference
pubmed:abstractText
Fission yeast pap1+ gene encodes an AP-1-like transcription factor, whose overexpression can confer resistance to staurosporine, a protein kinase inhibitor. We have previously identified a target gene (p25) for pap1+, and shown that, crm1+, which is required for maintenance of higher order chromosome structure, negatively regulates pap1-dependent transcription. In this study, we have characterized a novel gene, pad1+, which was isolated as a multicopy plasmid capable of conferring staurosporine-resistance. We showed that high copy pad1+ induces transcriptional activation of the p25 gene and that the induction by pad1+ is dependent on the pap1+ gene. Furthermore, a cis-element analysis of the 5'-region of the p25 gene showed that two elements (an AP-1 site and a 14 bp palindrome sequence) where pap1 binds in vitro is essential for the induction by pad1+. These results indicate that pad1 can positively regulate pap1-dependent transcription. Through an electromobility shift assay we showed that overexpression of pad1+ is not capable of enhancing the DNA-binding activity of pap1 directly. The pad1+ gene encodes a 35 kDa protein that has significant identity (68%) to Caenorhabditis elegans F37A4.5, and is also similar to mouse Mov34 and human C6.1A. Gene disruption experiments have demonstrated that pad1+ is essential for viability. A disruption mutant of pad1+ obtained after spore germination exhibited an elongated cell body with abberantly folded chromosomes. A mitotic plasmid loss experiment also produced similar cells having an abnormal chromosome structure. These suggest that pad1+ may play an important role in higher order chromosome structure. Taken concurrently with our previous results, two essential genes pad1+ and crm1+ regulate pap1-dependent transcription; pad1+ and crm1+ are positive and negative regulators, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
108 ( Pt 2)
pubmed:geneSymbol
p25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
569-79
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7769002-Amino Acid Sequence, pubmed-meshheading:7769002-Animals, pubmed-meshheading:7769002-Base Sequence, pubmed-meshheading:7769002-Caenorhabditis elegans, pubmed-meshheading:7769002-Chromosomes, Fungal, pubmed-meshheading:7769002-Fungal Proteins, pubmed-meshheading:7769002-Gene Expression Regulation, Fungal, pubmed-meshheading:7769002-Genes, Fungal, pubmed-meshheading:7769002-Karyopherins, pubmed-meshheading:7769002-Molecular Sequence Data, pubmed-meshheading:7769002-Phenotype, pubmed-meshheading:7769002-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:7769002-Schizosaccharomyces, pubmed-meshheading:7769002-Schizosaccharomyces pombe Proteins, pubmed-meshheading:7769002-Sequence Homology, Amino Acid, pubmed-meshheading:7769002-Trans-Activators, pubmed-meshheading:7769002-Transcription, Genetic, pubmed-meshheading:7769002-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
A novel essential fission yeast gene pad1+ positively regulates pap1(+)-dependent transcription and is implicated in the maintenance of chromosome structure.
pubmed:affiliation
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't