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rdf:type |
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lifeskim:mentions |
umls-concept:C0003805,
umls-concept:C0010762,
umls-concept:C0017337,
umls-concept:C0029939,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0171961,
umls-concept:C0205107,
umls-concept:C0439596,
umls-concept:C0534137,
umls-concept:C0851285,
umls-concept:C1417838,
umls-concept:C2698421
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pubmed:issue |
22
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pubmed:dateCreated |
1995-7-5
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pubmed:databankReference |
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pubmed:abstractText |
Aromatase P450, which is responsible for the metabolism of C19 steroids to estrogens, is expressed in the pre-ovulatory follicles and corpora lutea of ovulatory women by means of a promoter proximal to the start of translation (PII). To understand how this transcription is controlled by cAMP, we constructed chimeric constructs containing deletion mutations of the proximal promoter 5'-flanking DNA fused to the rabbit beta-globin reporter gene. Assay of reporter gene transcription in transfected bovine granulosa and luteal cells revealed that cAMP-stimulated transcription was lost upon deletion from -278 to -100 base pairs, indicating the presence of a functional cAMP-responsive element in this region; however, no classical cAMP-responsive element was found. Mutation of an AGGTCA motif located at -130 base pairs revealed that this element is crucial for cAMP-stimulated reporter gene transcription. When a single copy of this element was placed upstream of a heterologous promoter, it could act as a weak cAMP-response element. Supershift electrophoretic mobility shift assay and UV cross-linking established that Ad4BP/SF-1 binds to this hexameric element. Ad4BP/SF-1 mRNA and protein levels and DNA binding activity are increased in forskolin-treated luteal cells. We conclude that cAMP-stimulated transcription of human aromatase P450 in the ovary is due, at least in part, to increased levels and DNA binding activity of Ad4BP/SF-1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
270
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pubmed:geneSymbol |
CYP19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13561-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7768959-Animals,
pubmed-meshheading:7768959-Aromatase,
pubmed-meshheading:7768959-Base Sequence,
pubmed-meshheading:7768959-Cattle,
pubmed-meshheading:7768959-Cells, Cultured,
pubmed-meshheading:7768959-Cyclic AMP,
pubmed-meshheading:7768959-DNA,
pubmed-meshheading:7768959-DNA-Binding Proteins,
pubmed-meshheading:7768959-Female,
pubmed-meshheading:7768959-Fushi Tarazu Transcription Factors,
pubmed-meshheading:7768959-Homeodomain Proteins,
pubmed-meshheading:7768959-Humans,
pubmed-meshheading:7768959-Molecular Sequence Data,
pubmed-meshheading:7768959-Ovary,
pubmed-meshheading:7768959-Promoter Regions, Genetic,
pubmed-meshheading:7768959-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:7768959-Steroidogenic Factor 1,
pubmed-meshheading:7768959-Transcription, Genetic,
pubmed-meshheading:7768959-Transcription Factors
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pubmed:year |
1995
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pubmed:articleTitle |
Ad4BP/SF-1 regulates cyclic AMP-induced transcription from the proximal promoter (PII) of the human aromatase P450 (CYP19) gene in the ovary.
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pubmed:affiliation |
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235-9051, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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