Linked Life Data

7768196

Source:http://linkedlifedata.com/resource/pubmed/id/7768196

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-7-5
pubmed:abstractText
The coiled body is an ubiquitous nuclear organelle that contains essential components of the pre-mRNA splicing machinery as well as the nucleolar protein fibrillarin. Here we have studied the biogenesis of the coiled body in early mouse embryos. The results show that coiled bodies form and concentrate splicing snRNPs as early as in the maternal and paternal pronuclei of 1-cell embryos. This argues that the coiled body is likely to play a basic role in the nucleus of mammalian cells. In order to correlate the appearance of coiled bodies with the onset of transcriptional activity, embryos were incubated with brominated UTP and the incorporated nucleotide was visualized by fluorescence microscopy. In agreement with previous studies, transcriptional activity was first observed during the 2-cell stage. Thus, coiled bodies form before activation of embryonic gene expression. The appearance of coiled bodies in 1-cell embryos was preceded by the formation of morphologically distinct structures that also contain coilin and which we therefore refer to as pre-coiled bodies. At the electron microscopic level pre-coiled bodies have a compact fibrillar structure, whereas coiled bodies resemble a tangle of coiled threads. Although both pre-coiled bodies and coiled bodies contain the nucleolar protein fibrillarin, the assembly of coiled bodies is separated both in time and in space from ribosome synthesis. Our results suggest that the embryonic 'nucleolus-like body' is a structural scaffold that nucleates independently the formation of the coiled body and the assembly of the machinery responsible for ribosome biosynthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
601-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7768196-Animals, pubmed-meshheading:7768196-Cell Nucleus, pubmed-meshheading:7768196-Chromosomal Proteins, Non-Histone, pubmed-meshheading:7768196-Cleavage Stage, Ovum, pubmed-meshheading:7768196-Fluorescent Antibody Technique, pubmed-meshheading:7768196-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:7768196-In Situ Hybridization, pubmed-meshheading:7768196-Mice, pubmed-meshheading:7768196-Mice, Mutant Strains, pubmed-meshheading:7768196-Microscopy, Immunoelectron, pubmed-meshheading:7768196-Morphogenesis, pubmed-meshheading:7768196-Nuclear Proteins, pubmed-meshheading:7768196-Organelles, pubmed-meshheading:7768196-Phosphoproteins, pubmed-meshheading:7768196-RNA, Heterogeneous Nuclear, pubmed-meshheading:7768196-Ribonucleoproteins, pubmed-meshheading:7768196-Ribonucleoproteins, Small Nuclear, pubmed-meshheading:7768196-Transcription, Genetic, pubmed-meshheading:7768196-Zygote
pubmed:year
1995
pubmed:articleTitle
The biogenesis of the coiled body during early mouse development.
pubmed:affiliation
Institute of Histology and Embryology, Faculty of Medicine, University of Lisbon, Portugal.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't