Linked Life Data

7766706

Source:http://linkedlifedata.com/resource/pubmed/id/7766706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-7-6
pubmed:abstractText
Leukotriene (LT) C4 synthase catalyzes the conjugation of LTA4 with reduced glutathione (GSH) to form LTC4. This enzyme was purified to homogeneity from Kirsten Sarcoma transformed murine mast cells and from the human monocytic cell line THP-1 by two steps: a microsomal extract was partially purified by affinity chromatography on S-hexyl GSH agarose. LTC4 synthase was separated from other GSH-binding proteins by preparative gel electrophoresis under non denaturing conditions. The proteins obtained from human and murine cells showed a single band on a SDS gel with a molecular mass of 14 to 17 kDa, depending on the gel system. N-terminal sequencing revealed high homology between the LTC4 synthases of both species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
1256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Two step purification of human and murine leukotriene C4 synthase.
pubmed:affiliation
Medical Clinic IV, University of Erlangen-Nuernberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't