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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-4-15
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pubmed:abstractText |
Human pro-urokinase (pro-UK) gene was engineered for expression in mammalian cells. The stability of recombinant pro-UKs produced by two kinds of cells, Chinese hamster ovary (CHO) and human lymphoblastoid Namalwa KJM-1 cells, were compared. The pro-UK expressed in CHO cells in serum-free medium was degraded by cysteine endopeptidase secreted by CHO cells. This endopeptidase was inhibited by p-chloromercuribenzonate (PCMB) and leupeptin more efficiently than by aprotinin. On the other hand, the pro-UK expressed in Namalwa KJM-1 cells was not degraded, resulting in the stable production of pro-UK at a rate of 2-3 micrograms/10(6) cells/day by use of a gene amplification method with dihydrofolate reductase (DHFR) in serum-free medium. Thus, Namalwa KJM-1 cells showed the desired characteristics as a host cell for the production of recombinant proteins. The stability of recombinant proteins in heterologous systems may vary depending on the host cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
B
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0920-9069
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:geneSymbol |
pro-UK
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-88
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7764581-Amino Acid Sequence,
pubmed-meshheading:7764581-Animals,
pubmed-meshheading:7764581-Base Sequence,
pubmed-meshheading:7764581-CHO Cells,
pubmed-meshheading:7764581-Cell Line,
pubmed-meshheading:7764581-Cloning, Molecular,
pubmed-meshheading:7764581-Cricetinae,
pubmed-meshheading:7764581-Culture Media, Conditioned,
pubmed-meshheading:7764581-DNA,
pubmed-meshheading:7764581-Enzyme Precursors,
pubmed-meshheading:7764581-Enzyme Stability,
pubmed-meshheading:7764581-Humans,
pubmed-meshheading:7764581-Lymphocytes,
pubmed-meshheading:7764581-Molecular Sequence Data,
pubmed-meshheading:7764581-Recombinant Proteins,
pubmed-meshheading:7764581-Substrate Specificity,
pubmed-meshheading:7764581-Urokinase-Type Plasminogen Activator
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pubmed:year |
1993
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pubmed:articleTitle |
Stable production of recombinant pro-urokinase by human lymphoblastoid Namalwa KJM-1 cells: host-cell dependency of the expressed-protein stability.
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pubmed:affiliation |
Tokyo Research Laboratories, Kyowa Hakko Co., Ltd., Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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