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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1994-2-1
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pubmed:abstractText |
The effect of a fungal elicitor obtained from Alternaria sp. on growth and solasodine production by free and alginate-entrapped cells of Solanum eleagnifolium Cav. was studied. Fourteen-day-old cultures were elicited with 1% FW/V autoclaved homogenates. The solasodine production increased from 0.9 to 1.5 mg g-1 DW (65%) in suspension cultures and from 0.75 to 1.4 mg g-1 DW (about 95%) in entrapped cells. The maximum accumulation was obtained after 72 h of elicitation. In order to induce alkaloid release from cells (suspension and entrapped cells), permeabilization with 10% dimethylsulfoxide (DMSO) for 30 min was used. In both cases (free and entrapped cells), about 50-60% of intracellular solasodine was released into the medium. The reuse of elicited and permeabilized entrapped cells was also carried out for three production cycles.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
B
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0141-0229
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1074-7
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:7764297-Alternaria,
pubmed-meshheading:7764297-Cell Extracts,
pubmed-meshheading:7764297-Cell Membrane Permeability,
pubmed-meshheading:7764297-Cells, Cultured,
pubmed-meshheading:7764297-Dimethyl Sulfoxide,
pubmed-meshheading:7764297-Plants, Medicinal,
pubmed-meshheading:7764297-Solanaceous Alkaloids
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pubmed:year |
1993
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pubmed:articleTitle |
Effect of elicitation on the accumulation of solasodine by immobilized cells of Solanum eleagnifolium Cav.
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pubmed:affiliation |
Biotecnologia y Microbiologia Industrial, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
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pubmed:publicationType |
Journal Article
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