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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-11-24
|
| pubmed:abstractText |
Changes in the growth rate of Escherichia coli K12 J62-1 in response to the presence of plasmid pBR322 have been investigated. Plasmid-free and plasmid-containing strains were grown in batch culture and their maximum specific growth rate (mu max) determined. The acquisition of pBR322 by the host resulted in a decreased mu max. Following repeated subculturing of the plasmid-containing strain on selective medium, restoration in mu max was observed. The copy number and structure of the plasmid were not significantly altered during the experiment. Growth rate measurements for a series of strains constructed using a combination of host cells and plasmids with and without culture histories, indicated that the site of the adaptive mutation was located on the host chromosome rather than on the plasmid.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
B
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0266-8254
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
139-43
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1993
|
| pubmed:articleTitle |
Adaptation of Escherichia coli growth rates to the presence of pBR322.
|
| pubmed:affiliation |
Biology Division, School of Sciences, Staffordshire University, Stoke-on-Trent, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|