Linked Life Data

7763386

Source:http://linkedlifedata.com/resource/pubmed/id/7763386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-3-12
pubmed:databankReference
pubmed:abstractText
A Bacillus brevis gene coding for an endo-(1,3-1,4)-beta-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the beta-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3-1,4)-beta-glucanases. The optimum temperature and pH for enzyme activity were 65-70 degrees C and 8-10, respectively. When held at 75 degrees C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:geneSymbol
bglBB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
507-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4) beta-glucanase-encoding gene from an alkalophilic Bacillus brevis.
pubmed:affiliation
Biotechnology Programme, CSIR, Pretoria, Republic of South Africa.
pubmed:publicationType
Journal Article