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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1995-6-29
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pubmed:abstractText |
The S-form lipopolysaccharide (LPS) was effectively separated from a native preparation of smooth-type Salmonella abortus equi LPS by means of the centrifugal partition chromatography (CPC). To clarify the mechanisms by which LPS activates macrophages, CPC-separated S-form LPS was assessed for its ability to induce the secretion of tumor necrosis factor-alpha (TNF-alpha) by murine macrophage-like J774.1 cells in comparison with other fractions of LPS which lacks most of O-polysaccharides. LPS dose-response and time-kinetics studies showed that serum factor(s) regulated especially the onset of TNF-alpha secretion in stimulation with S-form LPS. These results strongly suggest that the native (unfractionated) LPS activates macrophages in both O-polysaccharide/serum-dependent and -independent pathways.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
25
|
pubmed:volume |
210
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
678-85
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7763241-Animals,
pubmed-meshheading:7763241-Cell Line,
pubmed-meshheading:7763241-Centrifugation,
pubmed-meshheading:7763241-Chromatography,
pubmed-meshheading:7763241-Dose-Response Relationship, Drug,
pubmed-meshheading:7763241-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7763241-Kinetics,
pubmed-meshheading:7763241-Lipopolysaccharides,
pubmed-meshheading:7763241-Macrophage Activation,
pubmed-meshheading:7763241-Macrophages,
pubmed-meshheading:7763241-Mice,
pubmed-meshheading:7763241-Molecular Weight,
pubmed-meshheading:7763241-Salmonella,
pubmed-meshheading:7763241-Tumor Necrosis Factor-alpha
|
pubmed:year |
1995
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pubmed:articleTitle |
Macrophage activation in response to S-form lipopolysaccharides (LPS) separated by centrifugal partition chromatography from wild-type LPS: effects of the O-polysaccharide portion of LPS.
|
pubmed:affiliation |
Faculty of Science, Osaka University, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|