|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0041637,
umls-concept:C0086418,
umls-concept:C0162638,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0225336,
umls-concept:C0376515,
umls-concept:C1366557,
umls-concept:C1704256,
umls-concept:C2911684
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|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1995-6-29
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|
pubmed:abstractText |
Transforming growth factor beta 1 (TGF-beta 1) is a potent growth inhibitor of human umbilical vein endothelial cells (HUVEC). We investigated whether apoptosis is involved in the TGF-beta 1 mediated HUVEC growth inhibition. Using immunofluorescence microscopy and staining by propidium iodide, we showed HUVEC treated with TGF-beta 1 contained characteristic apoptotic nuclei with condensation of chromatin. FACS analysis demonstrated that the number of cells with hypodiploid DNA contents were increased in HUVEC treated with TGF-beta 1. The DNA fragmentation assay exhibited typical ladder formation in TGF-beta 1-treated HUVEC. Using Western blot analysis, the expression of bcl-2 on HUVEC was down-regulated by TGF-beta 1-treatment. These findings suggest that TGF-beta 1 regulates the growth of HUVEC by inducing apoptosis accompanied with down-regulation of bcl-2 expression.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
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pubmed:month |
May
|
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pubmed:issn |
0006-291X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
25
|
|
pubmed:volume |
210
|
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pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
1076-82
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7763236-Apoptosis,
pubmed-meshheading:7763236-Cell Nucleus,
pubmed-meshheading:7763236-Cells, Cultured,
pubmed-meshheading:7763236-DNA,
pubmed-meshheading:7763236-DNA Damage,
pubmed-meshheading:7763236-Dose-Response Relationship, Drug,
pubmed-meshheading:7763236-Endothelium, Vascular,
pubmed-meshheading:7763236-Flow Cytometry,
pubmed-meshheading:7763236-Gene Expression Regulation,
pubmed-meshheading:7763236-Microscopy, Fluorescence,
pubmed-meshheading:7763236-Protein-Tyrosine Kinases,
pubmed-meshheading:7763236-Proto-Oncogene Proteins,
pubmed-meshheading:7763236-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:7763236-Transforming Growth Factor beta,
pubmed-meshheading:7763236-Umbilical Veins
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|
pubmed:year |
1995
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|
pubmed:articleTitle |
Transforming growth factor beta 1 induces apoptotic cell death in cultured human umbilical vein endothelial cells with down-regulated expression of bcl-2.
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|
pubmed:affiliation |
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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