Linked Life Data

7762443

Source:http://linkedlifedata.com/resource/pubmed/id/7762443

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-6-23
pubmed:abstractText
Fibroblast cells are known to have an ability to synthesize and secrete nerve growth factor (NGF). To investigate the mechanism of action of the iron-binding protein, lactoferrin (Lf), on cultured animal cells, the effect of bovine Lf (bLf) on NGF synthesis/secretion in mouse fibroblast cells was examined. Both apo- and holo-bLf induced an increase in NGF content in the cell-conditioned medium(CM) of mouse L-M cells, a line derived from L929 fibroblast cells, with similar effectiveness. The increase in NGF content in the CM of L-M cells cultured with bLf was not dependent on the induction of increase in cell numbers, but was due to induction of de novo synthesis of NGF in individual cells by bLf. Human Lf(hLf) also increased NGF content. However, apo- and holo-bovine transferrin (bTf) failed to stimulate the NGF synthesis. The time-dependent induction of NGF in L-M cells by bLf was different from that induced by basic fibroblast growth factor (bFGF) and bLf showed an additive effect with bFGF. These results suggest that the induction of NGF synthesis depends on a mechanism different from iron transport or bFGF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
357
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-85
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Lactoferrin promotes nerve growth factor synthesis/secretion in mouse fibroblast L-M cells.
pubmed:affiliation
Nutritional Science Laboratory, Morinaga Milk Industry Co., LTD, Kanagawa-Pref.
pubmed:publicationType
Journal Article