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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1995-6-29
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pubmed:databankReference | |
pubmed:abstractText |
Daudi, a lymphoblastoid B cell line derived from an African Burkitt lymphoma does not express HLA-A,B,C antigens at the cell surface. Although HLA-A,B,C heavy chains are made normally they do not assemble into functional molecules because beta 2-microglobulin is absent. Previous serological analysis of somatic cell hybrids indicated that the HLA haplotypes of Daudi encoded HLA-A1, A10(A26), B17, and B16(38) antigens. Here we describe the application of molecular methods: ARMS-PCR, cDNA cloning and sequencing, immunoprecipitation and gel electrophoresis, to define the class I genotype of the Daudi cell line which is HLA-A*0102, A*6601, B*5801, B*5802, Cw*0302 and Cw*0602. With the exception of the B38 antigen, which is not a product of the alleles defined, the genotype is consistent with the serological description. Two previously undiscovered alleles emerged from this analysis: A*0102 and B*5802. The A*0102 allele differs from A*0101 by 5 nucleotide substitutions within exon 2 where it has a motif shared with A*30 alleles; the B*5802 allele differs from B*5801 by 3 substitutions in exon 3 where it has a motif shared with B*14 alleles. Subtyping HLA-A1 alleles showed A*0102 was well represented amongst individuals typed serologically as A1 in an African population but was absent from caucasoids. B*5802 has been found in a second individual. Thus the novel A and B alleles are not specific to the Daudi tumor. Overall, this analysis of a single East African cell illustrates the power of molecular methods to define new class I HLA alleles in non-caucasoid populations.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0001-2815
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
177-87
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7761977-Africa, Eastern,
pubmed-meshheading:7761977-African Continental Ancestry Group,
pubmed-meshheading:7761977-Alleles,
pubmed-meshheading:7761977-Base Sequence,
pubmed-meshheading:7761977-DNA,
pubmed-meshheading:7761977-Gene Frequency,
pubmed-meshheading:7761977-Genes, MHC Class I,
pubmed-meshheading:7761977-HLA-A Antigens,
pubmed-meshheading:7761977-HLA-B Antigens,
pubmed-meshheading:7761977-HLA-C Antigens,
pubmed-meshheading:7761977-Humans,
pubmed-meshheading:7761977-Molecular Sequence Data,
pubmed-meshheading:7761977-Polymerase Chain Reaction,
pubmed-meshheading:7761977-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles.
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pubmed:affiliation |
Cancer Immunology Laboratory, ICRF, John Radcliffe Hospital, Oxford, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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