rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
6
|
pubmed:dateCreated |
1995-6-28
|
pubmed:abstractText |
Retroviruses and long terminal repeat (LTR)-containing retrotransposons initiate reverse transcription by using a specific tRNA primer than anneals to the primer-binding site of the retroelement transcript. Sequences from a large number of retroviruses and LTR-containing retrotransposons had indicated that the role of tRNAs in priming reverse transcription is universal among these LTR-containing retroelements. Data presented here strongly support the surprising conclusion that Tf1, a highly active LTR-containing retrotransposon isolated from Schizosaccharomyces pombe, undergoes a novel self-priming process that requires hybridization between the primer-binding site and the first 11 bases of the Tf1 transcript. Single-base mutations in these regions block transposition and reverse transcription, while compensatory mutations that reestablish complementarily rescue both defects. In addition, the sequence of the minus-strand RNA primer of reverse transcription was consistent with its being derived from the 5' end of the Tf1 transcript. Evidence that this mechanism defines a new family of retroelements is presented.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1312461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1314382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1377773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1378431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1384989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1846969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2005825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2159935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2174117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2411424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2430190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2465091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2843295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-3323810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7505202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7506345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7528326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7679954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8223497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8389039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8396547
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0270-7306
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
15
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3310-7
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
|
pubmed:year |
1995
|
pubmed:articleTitle |
A novel mechanism of self-primed reverse transcription defines a new family of retroelements.
|
pubmed:affiliation |
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA.
|
pubmed:publicationType |
Journal Article
|