Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-6-28
pubmed:abstractText
Retroviruses and long terminal repeat (LTR)-containing retrotransposons initiate reverse transcription by using a specific tRNA primer than anneals to the primer-binding site of the retroelement transcript. Sequences from a large number of retroviruses and LTR-containing retrotransposons had indicated that the role of tRNAs in priming reverse transcription is universal among these LTR-containing retroelements. Data presented here strongly support the surprising conclusion that Tf1, a highly active LTR-containing retrotransposon isolated from Schizosaccharomyces pombe, undergoes a novel self-priming process that requires hybridization between the primer-binding site and the first 11 bases of the Tf1 transcript. Single-base mutations in these regions block transposition and reverse transcription, while compensatory mutations that reestablish complementarily rescue both defects. In addition, the sequence of the minus-strand RNA primer of reverse transcription was consistent with its being derived from the 5' end of the Tf1 transcript. Evidence that this mechanism defines a new family of retroelements is presented.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1312461, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1314382, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1377773, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1378431, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1384989, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-1846969, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2005825, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2159935, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2174117, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2411424, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2430190, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2465091, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-2843295, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-3323810, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7505202, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7506345, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7528326, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-7679954, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8223497, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8389039, http://linkedlifedata.com/resource/pubmed/commentcorrection/7760826-8396547
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3310-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A novel mechanism of self-primed reverse transcription defines a new family of retroelements.
pubmed:affiliation
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article