Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1995-6-27
pubmed:databankReference
pubmed:abstractText
Transcriptional control of steroid-regulated gene networks by nuclear receptor proteins results in the coordinate expression of a limited number of target genes. Although much is known about the structure and function of steroid receptors, relatively few cell-specific steroid-regulated genes have been isolated and characterized. In this paper we describe results using mRNA differential display reverse transcriptase PCR (DDPCR) to identify and isolate short cDNA sequence tags from thymocyte and prostate cells under various hormone conditions. Using this technique we have isolated several differentially expressed sequence tags (DESTs) from the mouse thymocyte cell line WEHI 7.2. Two of these DESTs, GIG10 and GIG18, are rapidly induced by dexamethasone within 2 h of treatment. GIG10 is a novel sequence and GIG18 is the mouse homologue of a human expressed sequence tag isolated from activated B lymphocytes. We also used DDPCR to isolate DESTs from androgen-modulated rat ventral prostate tissue, one of which we characterized and found to correspond to the 3' end of prostatic spermine binding protein mRNA, a known androgen-regulated gene. Modifications of the original DDPCR protocol, which we found can potentially decrease the frequency of isolating false-positive DESTs, are described and the merits of DDPCR, relative to other differential cDNA cloning strategies, are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
R1-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7758820-Androgens, pubmed-meshheading:7758820-Animals, pubmed-meshheading:7758820-Base Sequence, pubmed-meshheading:7758820-Blotting, Northern, pubmed-meshheading:7758820-Cell Line, pubmed-meshheading:7758820-Cloning, Molecular, pubmed-meshheading:7758820-DNA, pubmed-meshheading:7758820-Dexamethasone, pubmed-meshheading:7758820-Humans, pubmed-meshheading:7758820-Lymphocytes, pubmed-meshheading:7758820-Male, pubmed-meshheading:7758820-Mice, pubmed-meshheading:7758820-Molecular Sequence Data, pubmed-meshheading:7758820-Polymerase Chain Reaction, pubmed-meshheading:7758820-Prostate, pubmed-meshheading:7758820-RNA, Messenger, pubmed-meshheading:7758820-Rats, pubmed-meshheading:7758820-Receptors, Glucocorticoid, pubmed-meshheading:7758820-Sequence Tagged Sites, pubmed-meshheading:7758820-Thymus Gland
pubmed:year
1995
pubmed:articleTitle
Isolation of differentially expressed sequence tags from steroid-responsive cells using mRNA differential display.
pubmed:affiliation
Department of Biochemistry, University of Arizona, Tucson 85724, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't