Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-6-27
pubmed:abstractText
Distribution of actin in spermatozoa before (prospermia) and after (spermiophores) cell eversion process in a soft tick, Argas (A.) polonicus, was studied by phalloidin-rhodamine fluorescence and immunogold labelling methods. F-actin was mainly localized at the cortical cytoplasm surrounding the vacuole in prospermia and along the periphery of spermiophores. A further localization of actin was in the cytoplasm in the "head" and adjacent region both in prospermium and spermiophore. Immunogold labelling revealed the presence of actin in the cytoplasm of whole spermatozoon with higher concentration in the "head" region. In the peripheral cytoplasm, however, actin was found in the material coating electron-dense fibres. The fibres themselves and cell protrusions were not labelled suggesting that F-actin was localized around dense fibres. Of two distinct pools of F-actin found in the tick spermatozoa, the one located in the subplasmalemmal cytoplasm is supposedly responsible for the gliding movement, whereas that filling the anterior tapered end including "head"--may be involved in contortional movements of that region. The participation of actin-based mechanism in forward gliding of spermiophores was confirmed in experiments with cytochalasin B. The contribution of actin to prospermium vacuole opening and subsequent cell eversion is suggested.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0239-8508
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-64
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Actin in spermatozoon of a soft tick, Argas (A.) polonicus (Ixodida, Acari).
pubmed:affiliation
Department of Comparative Anatomy, Jagiellonian University, Kraków, Poland.
pubmed:publicationType
Journal Article