Linked Life Data

7755971

Source:http://linkedlifedata.com/resource/pubmed/id/7755971

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-6-27
pubmed:abstractText
In vivo recombination as a tool for plasmid construction was analyzed using a model system based on the properties of the RecF pathway in Escherichia coli. This pathway was used after a double strand break (DSB) effected by restriction enzyme cleavage of the plasmids of interest. DSB repair was shown to be independent of the methylation state of the vector or insert DNA, of the type of restriction enzyme used (5' or 3' overhanging or blunt ends) and of dephosphorylation of the vector and/or template. Since the E. coli repair system does not recognize insertions, this recombination mechanism can be used to exchange cDNAs between different vectors. Some implications of the results on the mechanism of recombination are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-7
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Evaluation of Escherichia coli recBC sbcBC mutants for cloning by recombination in vivo.
pubmed:affiliation
Yeast Genetics Department, Transgène SA, Strasbourg, France.
pubmed:publicationType
Journal Article