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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-6-27
|
| pubmed:abstractText |
Studies on the molecular basis of neurological and psychiatric disorders often rely on the precise determination of specific proteins in brain tissues. In this study, we have developed a method for measuring the levels of the neural-specific growth-associated protein, GAP-43, in human postmortem brain specimens. This rapid and quantitative method is based on immunodetection procedures. Briefly, synaptosomal plasma membranes (SPMs) are deposited onto polyvinylidene difluoride (PVDF) membranes via a dot-blotting apparatus, followed by specific GAP-43 detection using a monospecific polyclonal antibody. Overall, the dot-blot procedure provided several advantages over Western blots and one-dimensional and two-dimensional polyacrylamide gels. The assays were more sensitive, reproducible, and allowed the rapid and simultaneous determination of multiple samples. Using this technique, we examined the levels of the GAP-43 protein in Brodmann's areas 17, 20, and 10 of schizophrenic and age-, sex-, and postmortem interval (PMI) matched controls. These studies revealed an increase in the levels of GAP-43 in visual association and frontal cortices (areas 20 and 10) of schizophrenic brains. Given the relationship of GAP-43 expression with the establishment and remodeling of neural connections, our results support the hypothesis that schizophrenia is associated with a perturbed organization of synaptic connections in associative areas of the human brain.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Haloperidol,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurofilament Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1044-7393
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-11
|
| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:7755843-Adult,
pubmed-meshheading:7755843-Aged,
pubmed-meshheading:7755843-Animals,
pubmed-meshheading:7755843-Blotting, Western,
pubmed-meshheading:7755843-Brain,
pubmed-meshheading:7755843-Brain Chemistry,
pubmed-meshheading:7755843-Cell Membrane,
pubmed-meshheading:7755843-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:7755843-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7755843-Female,
pubmed-meshheading:7755843-GAP-43 Protein,
pubmed-meshheading:7755843-Haloperidol,
pubmed-meshheading:7755843-Humans,
pubmed-meshheading:7755843-Immunoblotting,
pubmed-meshheading:7755843-Male,
pubmed-meshheading:7755843-Membrane Glycoproteins,
pubmed-meshheading:7755843-Middle Aged,
pubmed-meshheading:7755843-Nerve Tissue Proteins,
pubmed-meshheading:7755843-Neurofilament Proteins,
pubmed-meshheading:7755843-Postmortem Changes,
pubmed-meshheading:7755843-Rats,
pubmed-meshheading:7755843-Rats, Sprague-Dawley,
pubmed-meshheading:7755843-Reference Values,
pubmed-meshheading:7755843-Reproducibility of Results,
pubmed-meshheading:7755843-Schizophrenia,
pubmed-meshheading:7755843-Sensitivity and Specificity,
pubmed-meshheading:7755843-Synaptosomes,
pubmed-meshheading:7755843-Visual Cortex
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Increased levels of GAP-43 protein in schizophrenic brain tissues demonstrated by a novel immunodetection method.
|
| pubmed:affiliation |
Department of Chemistry, University of New Mexico School of Medicine, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|