Linked Life Data

7755662

Source:http://linkedlifedata.com/resource/pubmed/id/7755662

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-12-21
pubmed:abstractText
Unique virus-like tubulofilamentous particles, termed nemavirus, have been consistently observed in spongiform encephalopathic brains by electron microscopy in thin sections. Sodium dodecyl sulphate treatment of unfixed infected brain tissue on grids revealed a twisted fibril core of tubulofilamentous particles. The unmasked fibrils were identified as scrapie-associated fibrils by immunogold labelling. Both tubulofilamentous particles and scrapie-associated fibrils are not artefacts of protease treatment of protein formed in vitro during purification. Treatment with protease and nucleases revealed that each tubule consists of three layers: (i) an outer coat of protease-sensitive material; (ii) an intermediate layer that is digested by DNase and mung bean nuclease, and (iii) inner protease-resistant protein scrapie-associated fibril.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5526
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Evidence that scrapie-associated tubulofilamentous particles contain a single-stranded DNA.
pubmed:affiliation
Public Health Laboratory, Newcastle General Hospital, Newcastle-upon-Tyne, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't